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Kirrel3-Mediated Synapse Formation Is Attenuated by Disease-Associated Missense Variants
Journal article   Open access   Peer reviewed

Kirrel3-Mediated Synapse Formation Is Attenuated by Disease-Associated Missense Variants

Matthew R Taylor, E Anne Martin, Brooke Sinnen, Rajdeep Trilokekar, Emmanuelle Ranza, Stylianos E Antonarakis and Megan E Williams
The Journal of neuroscience, Vol.40(28), pp.5376-5388
07/08/2020
DOI: 10.1523/JNEUROSCI.3058-19.2020
PMCID: PMC7343328
PMID: 32503885
url
https://doi.org/10.1523/JNEUROSCI.3058-19.2020View
Published (Version of record) Open Access

Abstract

Missense variants in Kirrel3 are repeatedly identified as risk factors for autism spectrum disorder and intellectual disability, but it has not been reported if or how these variants disrupt Kirrel3 function. Previously, we studied Kirrel3 loss of function using KO mice and showed that Kirrel3 is a synaptic adhesion molecule necessary to form one specific type of hippocampal synapse Here, we developed an , gain-of-function assay for Kirrel3 using neuron cultures prepared from male and female mice and rats. We find that WT Kirrel3 induces synapse formation selectively between Kirrel3-expressing neurons via homophilic, transcellular binding. We tested six disease-associated Kirrel3 missense variants and found that five attenuate this synaptogenic function. All variants tested traffic to the cell surface and localize to synapses similar to WT Kirrel3. Two tested variants lack homophilic transcellular binding, which likely accounts for their reduced synaptogenic function. Interestingly, we also identified variants that bind in trans but cannot induce synapses, indicating that Kirrel3 transcellular binding is necessary but not sufficient for its synaptogenic function. Collectively, these results suggest Kirrel3 functions as a synaptogenic, cell-recognition molecule, and this function is attenuated by missense variants associated with autism spectrum disorder and intellectual disability. Thus, we provide critical insight to the mechanism of Kirrel3 function and the consequences of missense variants associated with autism and intellectual disability. Here, we advance our understanding of mechanisms mediating target-specific synapse formation by providing evidence that Kirrel3 transcellular interactions mediate target recognition and signaling to promote synapse development. Moreover, this study tests the effects of disease-associated Kirrel3 missense variants on synapse formation, and thereby, increases understanding of the complex etiology of neurodevelopmental disorders arising from rare missense variants in synaptic genes.
 Animals Autistic Disorder - genetics Autistic Disorder - metabolism Cell Adhesion - physiology Cells, Cultured Female Hippocampus - cytology Hippocampus - metabolism Intellectual Disability - genetics Intellectual Disability - metabolism Male Membrane Proteins - genetics Membrane Proteins - metabolism Mice Mutation, Missense Neurons - metabolism Rats Synapses - metabolism

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