Journal article
L-Galactose replaces L-fucose in the pectic polysaccharide rhamnogalacturonan II synthesized by the L-fucose-deficient mur1 Arabidopsis mutant
Planta, Vol.219(1), pp.147-157
2004
DOI: 10.1007/s00425-004-1205-x
PMID: 14991405
Abstract
Arabidopsis thaliana mur1 is a dwarf mutant with altered cell-wall properties, in which L-fucose is partially replaced by L-galactose in the xyloglucan and glycoproteins. We found that the mur1 mutation also affects the primary structure of the pectic polysaccharide rhamnogalacturonan II (RG-II). In mur1 RG-II a non-reducing terminal 2-O-methyl L-galactosyl residue and a 3,4-linked L-galactosyl residue replace the non-reducing terminal 2-O-methyl L-fucosyl residue and the 3,4-linked L-fucosyl residue, respectively, that are present in wild-type RG-II. Furthermore, we found that a terminal non-reducing L-galactosyl residue, rather than the previously reported D-galactosyl residue, is present on the 2-O-methyl xylose-containing side chain of RG-II in both wild type and mur1 plants. Approximately 95% of the RG-II from wild type and mur1 plants is solubilized as a high-molecular-weight (>100 kDa) complex, by treating walls with aqueous potassium phosphate. The molecular mass of RG-II in this complex was reduced to 5–10 kDa by treatment with endopolygalacturonase, providing additional evidence that RG-II is covalently linked to homogalacturonan. The results of this study provide additional information on the structure of RG-II and the role of this pectic polysaccharide in the plant cell wall.
Details
- Title: Subtitle
- L-Galactose replaces L-fucose in the pectic polysaccharide rhamnogalacturonan II synthesized by the L-fucose-deficient mur1 Arabidopsis mutant
- Creators
- Bradley L REUHS - Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, The University of Georgia, 315 Riverbend Road, Athens, GA 30602-4712, United StatesJoshua GLENN - Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, The University of Georgia, 315 Riverbend Road, Athens, GA 30602-4712, United StatesSamuel B STEPHENS - Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, The University of Georgia, 315 Riverbend Road, Athens, GA 30602-4712, United StatesJohn S KIM - Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, The University of Georgia, 315 Riverbend Road, Athens, GA 30602-4712, United StatesD. Benjamin CHRISTIE - Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, The University of Georgia, 315 Riverbend Road, Athens, GA 30602-4712, United StatesJohn G GLUSHKA - Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, The University of Georgia, 315 Riverbend Road, Athens, GA 30602-4712, United StatesEarl ZABLACKIS - Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, The University of Georgia, 315 Riverbend Road, Athens, GA 30602-4712, United StatesPeter ALBERSHEIM - Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, The University of Georgia, 315 Riverbend Road, Athens, GA 30602-4712, United StatesAlan G DARVILL - Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, The University of Georgia, 315 Riverbend Road, Athens, GA 30602-4712, United StatesMalcolm A O'NEILL - Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, The University of Georgia, 315 Riverbend Road, Athens, GA 30602-4712, United States
- Resource Type
- Journal article
- Publication Details
- Planta, Vol.219(1), pp.147-157
- Publisher
- Springer
- DOI
- 10.1007/s00425-004-1205-x
- PMID
- 14991405
- ISSN
- 0032-0935
- eISSN
- 1432-2048
- Language
- English
- Date published
- 2004
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094567102771
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