L-fucose is accumulated via a specific transport system in eukaryotic cells

Thomas J Wiese; Joyce A Dunlap; Mark A Yorek

doi:10.1016/S0021-9258(17)31703-9

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L-fucose is accumulated via a specific transport system in eukaryotic cells

Journal article

Open access

Peer reviewed

L-fucose is accumulated via a specific transport system in eukaryotic cells

Thomas J Wiese, Joyce A Dunlap and Mark A Yorek

The Journal of biological chemistry, Vol.269(36), pp.22705-22711

09/09/1994

DOI: 10.1016/S0021-9258(17)31703-9

PMID: 8077222

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Abstract

L-Fucose is a monosaccharide normally present at low concentrations in serum and is the only levorotatory sugar utilized by mammalian systems. The metabolism of L-fucose is only partially understood. In this report, we characterize the uptake of L-fucose by four widely varying mammalian cell lines (murine neuroblastoma, bovine aortic endothelial, murine cerebral microvessel endothelial, and Madin-Darby canine kidney cells). Based on the criteria of saturability and specificity of L-fucose uptake, we conclude that L-fucose is accumulated via a specific recognition mechanism. Accumulation of L-fucose at 4 degrees C and in the presence of colchicine and cytochalasin D rules out receptor-mediated endocytosis as an uptake mechanism. Thus, the accumulation appears to be via a carrier system. Using a variety of criteria, we determined that L-fucose is not taken up by a glucose transporter system. Accumulation of L-[5,6-3H]fucose is Na(+)-independent and reduced by loading cells with L-fucose or depleting the cell of its phosphorylation capability, suggesting that the uptake of L-fucose is by passive facilitative diffusion. A significant amount of the L-fucose taken up by each of the four cell types was incorporated into protein and secreted into the medium.

Cell Line

Cytochalasin B - pharmacology

Deoxyglucose - metabolism

Fucose - metabolism

Cells, Cultured

Cerebrovascular Circulation

Neuroblastoma

Kidney

Microcirculation

Animals

Phloretin - pharmacology

Cattle

Endothelium, Vascular - metabolism

Dogs

Aorta

Monosaccharides - pharmacology

Biological Transport - drug effects

Mice

Kinetics

Tumor Cells, Cultured

Diffusion

Phlorhizin - pharmacology

Details

Title: Subtitle: L-fucose is accumulated via a specific transport system in eukaryotic cells
Creators: Thomas J Wiese - Department of Internal Medicine, University of Iowa, Iowa City 52246
Joyce A Dunlap
Mark A Yorek
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.269(36), pp.22705-22711
DOI: 10.1016/S0021-9258(17)31703-9
PMID: 8077222
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Grant note: 45453 / PHS HHS 25295 / PHS HHS 07018 / PHS HHS
Language: English
Date published: 09/09/1994
Academic Unit: Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
Record Identifier: 9984094663202771

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39 Times Cited - Web of Science