Journal article
L-fucose reduces collagen and noncollagen protein production in cultured cerebral microvessel endothelial cells
Journal of cellular physiology, Vol.165(3), pp.658-666
12/1995
DOI: 10.1002/jcp.1041650325
PMID: 7593246
Abstract
L-fucose is a monosaccharide which is present in low concentrations in normal serum but is increased in diabetes, cancer, and inflammatory diseases. The contribution that abnormal L-fucose levels make to the progression of these disorders is unknown. In a previous study we showed that increased L-fucose concentration reduced proliferation and proteoglycan production by cultured cerebral microvessel endothelial cells. In the present study we show that exposing cerebral microvessel endothelial cells for 2 weeks to medium containing an increased concentration of L-fucose causes a significant decrease in collagen and to a lesser extent noncollagan protein production. The effect of L-fucose on collagen and noncollagen protein production is concentration-dependent: 1 mM L-fucose causes a significant decrease in collagen production but has no effect on noncollagen protein production; a 5mM L-fucose concentration causes a maximum decrease in both collagen and noncollagen protein production. This defect is unrelated to the reduction in myo-inositol uptake caused by L-fucose and is not prevented by aminoguanidine. Collagen production can be improved by restoring L-fucose-conditioned cells to normal medium. Culturing cells for 2 weeks in medium containing 10 mM L-fucose resulted in a 50% decrease in collagen production, which was restored to 75% of control after cells were transferred to normal medium for 7 days. In contrast, noncollagen protein production was totally restored after 3 days in normal medium. Increasing levels of L-fucose in serum of rats also resulted in a decrease in collagen production. Collagenase digestible incorporation of L-[2,3,4,5-3H]proline into protein of the articular cartilage from rats fed a diet containing 20% L-fucose for 3 weeks was reduced by about 40% compared to rats fed a normal diet. The decrease in collagen production in L-fucose fed rats was less than the reduction that occurred in streptozotocin-induced diabetic rats. These data suggest that changes in L-fucose concentration itself may be a factor in the regulation of collagen production.
Details
- Title: Subtitle
- L-fucose reduces collagen and noncollagen protein production in cultured cerebral microvessel endothelial cells
- Creators
- Mark A Yorek - Department of Internal Medicine, Veterans Affairs Medical Center, and Diabetes Endocrinology Research Center, University of Iowa, Iowa City, Iowa 52242Corinne E Conner - Department of Internal Medicine, Veterans Affairs Medical Center, and Diabetes Endocrinology Research Center, University of Iowa, Iowa City, Iowa 52242Robert G Spanheimer - Department of Internal Medicine, Veterans Affairs Medical Center, and Diabetes Endocrinology Research Center, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Journal of cellular physiology, Vol.165(3), pp.658-666
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/jcp.1041650325
- PMID
- 7593246
- ISSN
- 0021-9541
- eISSN
- 1097-4652
- Number of pages
- 9
- Language
- English
- Date published
- 12/1995
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094590102771
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