LAB-1 antagonizes the Aurora B kinase in C. elegans

Carlos Egydio de Carvalho; Sophie Zaaijer; Sarit Smolikov; Yanjie Gu; Jill M Schumacher; Monica P Colaiácovo

doi:10.1101/gad.1691208

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LAB-1 antagonizes the Aurora B kinase in C. elegans

Journal article

Open access

Peer reviewed

LAB-1 antagonizes the Aurora B kinase in C. elegans

Carlos Egydio de Carvalho, Sophie Zaaijer, Sarit Smolikov, Yanjie Gu, Jill M Schumacher and Monica P Colaiácovo

Genes & development, Vol.22(20), pp.2869-2885

10/15/2008

DOI: 10.1101/gad.1691208

PMCID: PMC2569883

PMID: 18923084

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Abstract

The Shugoshin/Aurora circuitry that controls the timely release of cohesins from sister chromatids in meiosis and mitosis is widely conserved among eukaryotes, although little is known about its function in organisms whose chromosomes lack a localized centromere. Here we show that Caenorhabditis elegans chromosomes rely on an alternative mechanism to protect meiotic cohesin that is shugoshin-independent and instead involves the activity of a new chromosome-associated protein named LAB-1 (Long Arm of the Bivalent). LAB-1 preserves meiotic sister chromatid cohesion by restricting the localization of the C. elegans Aurora B kinase, AIR-2, to the interface between homologs via the activity of the PP1/Glc7 phosphatase GSP-2. The localization of LAB-1 to chromosomes of dividing embryos and the suppression of mitotic-specific defects in air-2 mutant embryos with reduced LAB-1 activity support a global role of LAB-1 in antagonizing AIR-2 in both meiosis and mitosis. Although the localization of a GFP fusion and the analysis of mutants and RNAi-mediated knockdowns downplay a role for the C. elegans shugoshin protein in cohesin protection, shugoshin nevertheless helps to ensure the high fidelity of chromosome segregation at metaphase I. We propose that, in C. elegans, a LAB-1-mediated mechanism evolved to offset the challenges of providing protection against separase activity throughout a larger chromosome area.

Meiosis - physiology

Embryo, Nonmammalian - cytology

Chromatids - genetics

Embryo, Nonmammalian - metabolism

Caenorhabditis elegans Proteins - metabolism

Molecular Sequence Data

RNA, Messenger - metabolism

Chromatids - metabolism

Chromosomal Proteins, Non-Histone - antagonists & inhibitors

DNA-Binding Proteins - metabolism

Aurora Kinase B

Multiprotein Complexes - metabolism

RNA, Helminth - metabolism

Gene Expression Regulation, Developmental

Immunoglobulin G - immunology

Protein-Serine-Threonine Kinases - antagonists & inhibitors

Sister Chromatid Exchange

RNA, Helminth - genetics

Protein-Serine-Threonine Kinases - metabolism

Amino Acid Sequence

Chromosome Segregation

Chromosomal Proteins, Non-Histone - metabolism

RNA, Messenger - genetics

RNA, Small Interfering - pharmacology

Cell Cycle Proteins - metabolism

Protein-Serine-Threonine Kinases - genetics

Adenosine Triphosphatases - metabolism

Aurora Kinases

Meiotic Prophase I - physiology

Reverse Transcriptase Polymerase Chain Reaction

Chromosomal Proteins, Non-Histone - genetics

Sequence Homology, Amino Acid

Caenorhabditis elegans

Animals

Caenorhabditis elegans Proteins - antagonists & inhibitors

Mitosis - physiology

Fluorescent Antibody Technique

Caenorhabditis elegans Proteins - genetics

Details

Title: Subtitle: LAB-1 antagonizes the Aurora B kinase in C. elegans
Creators: Carlos Egydio de Carvalho - Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
Sophie Zaaijer
Sarit Smolikov - University of Iowa, Biology
Yanjie Gu
Jill M Schumacher
Monica P Colaiácovo
Resource Type: Journal article
Publication Details: Genes & development, Vol.22(20), pp.2869-2885
Publisher: United States
DOI: 10.1101/gad.1691208
PMID: 18923084
PMCID: PMC2569883
ISSN: 0890-9369
eISSN: 1549-5477
Grant note: R01 GM062181-07 / NIGMS NIH HHS R01 GM062181-08 / NIGMS NIH HHS R01GM072551 / NIGMS NIH HHS R01GM62181 / NIGMS NIH HHS R01 GM062181-06 / NIGMS NIH HHS R01 GM062181 / NIGMS NIH HHS R01 GM072551 / NIGMS NIH HHS
Language: English
Date published: 10/15/2008
Academic Unit: Biology
Record Identifier: 9983992060702771

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