Journal article
Label-Free, Flow-Imaging Methods for Determination of Cell Concentration and Viability
Pharmaceutical research, Vol.35(8), 150
08/01/2018
DOI: 10.1007/s11095-018-2422-5
PMCID: PMC5976703
PMID: 29846807
Abstract
Purpose
To investigate the potential of two flow imaging microscopy (FIM) techniques (Micro-Flow Imaging (MFI) and FlowCAM) to determine total cell concentration and cell viability.
Methods
B-lineage acute lymphoblastic leukemia (B-ALL) cells of 2 different donors were exposed to ambient conditions. Samples were taken at different days and measured with MFI, FlowCAM, hemocytometry and automated cell counting. Dead and live cells from a fresh B-ALL cell suspension were fractionated by flow cytometry in order to derive software filters based on morphological parameters of separate cell populations with MFI and FlowCAM. The filter sets were used to assess cell viability in the measured samples.
Results
All techniques gave fairly similar cell concentration values over the whole incubation period. MFI showed to be superior with respect to precision, whereas FlowCAM provided particle images with a higher resolution. Moreover, both FIM methods were able to provide similar results for cell viability as the conventional methods (hemocytometry and automated cell counting).
Conclusion
FIM-based methods may be advantageous over conventional cell methods for determining total cell concentration and cell viability, as FIM measures much larger sample volumes, does not require labeling, is less laborious and provides images of individual cells.
Details
- Title: Subtitle
- Label-Free, Flow-Imaging Methods for Determination of Cell Concentration and Viability
- Creators
- A. S. Sediq - Centre for Human Drug ResearchR. Klem - Centre for Human Drug ResearchM. R. Nejadnik - Centre for Human Drug ResearchP. Meij - Leiden University Medical CenterWim Jiskoot - Leiden University
- Resource Type
- Journal article
- Publication Details
- Pharmaceutical research, Vol.35(8), 150
- DOI
- 10.1007/s11095-018-2422-5
- PMID
- 29846807
- PMCID
- PMC5976703
- NLM abbreviation
- Pharm Res
- ISSN
- 0724-8741
- eISSN
- 1573-904X
- Grant note
- DOI: 10.13039/501100001717, name: Leiden University
- Language
- English
- Date published
- 08/01/2018
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984420846302771
Metrics
11 Record Views