Journal article
Label-Free Isolation and mRNA Detection of Circulating Tumor Cells from Patients with Metastatic Lung Cancer for Disease Diagnosis and Monitoring Therapeutic Efficacy
Analytical chemistry (Washington), Vol.87(23), pp.11893-11900
12/01/2015
DOI: 10.1021/acs.analchem.5b03484
PMID: 26531886
Abstract
We develop an inertial-based microfluidic cell sorter combined with an integrated membrane filter, allowing for size-based, label-free, and high-efficiency separation and enrichment of circulating tumor cells (CTCs) in whole blood. The cell sorter is composed of a double spiral microchannel that hydrodynamically focuses and separates large CTCs from small blood cells. The focused CTCs with the equilibrium position around the midline of microchannel are further captured and enriched by a membrane filter (pore size of 8 μm) attached at the middle outlet. This integrated microfluidic device can process 1 mL of whole blood containing spiked tumor cells (A549, human lung adenocarcinoma epithelial cell line) within 15 min, with the capture efficiency of 74.4% at the concentration as low as tens of A549 cells per mL of whole blood. This microfluidic cell sorter is further adopted for isolation of CTCs from peripheral blood samples of patients with metastatic lung cancer. The immunostaining and CK-19 mRNA detection are applied for identification of captured CTCs, showing that our method can detect 90% of metastatic lung cancer patients before therapy, whereas the commercially used system can only detect 40% of the same patients. We also use the expression of CK-19 mRNA from captured CTCs as an indicator for monitoring the therapeutic efficiency, which correlates well with X-ray computed tomography (CT) assessment of the disease.
Details
- Title: Subtitle
- Label-Free Isolation and mRNA Detection of Circulating Tumor Cells from Patients with Metastatic Lung Cancer for Disease Diagnosis and Monitoring Therapeutic Efficacy
- Creators
- Jidong Wang - National Center for Nanoscience and TechnologyWenjing Lu - National Center for Nanoscience and TechnologyChuanhao Tang - Academy of Military Medical SciencesYi Liu - Academy of Military Medical SciencesJiashu Sun - National Center for Nanoscience and TechnologyXuan Mu - Peking Union Medical College HospitalLin Zhang - Peking Union Medical College HospitalBo Dai - Fudan University Shanghai Cancer CenterXiaoyan Li - Academy of Military Medical SciencesHailong Zhuo - Academy of Military Medical SciencesXingyu Jiang - National Center for Nanoscience and Technology
- Resource Type
- Journal article
- Publication Details
- Analytical chemistry (Washington), Vol.87(23), pp.11893-11900
- DOI
- 10.1021/acs.analchem.5b03484
- PMID
- 26531886
- ISSN
- 0003-2700
- eISSN
- 1520-6882
- Grant note
- DOI: 10.13039/501100002855, name: Ministry of Science and Technology of the People's Republic of China, award: 2013AA032204, 2013YQ190467; DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: 21475028, 21535001, 81361140345; name: Beijing Municipal Commission of Science and Technology, award: Z131100002713024
- Language
- English
- Date published
- 12/01/2015
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984276452202771
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