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Label-free measurements of reaction kinetics using a droplet-based optofluidic device
Journal article   Open access

Label-free measurements of reaction kinetics using a droplet-based optofluidic device

Zhangming Mao, Feng Guo, Yuliang Xie, Yanhui Zhao, Michael Ian Lapsley, Lin Wang, John D Mai, Francesco Costanzo and Tony Jun Huang
Journal of laboratory automation, Vol.20(1), pp.17-24
02/2015
DOI: 10.1177/2211068214549625
PMID: 25249275
url
https://doi.org/10.1177/2211068214549625View
Published (Version of record) Open Access

Abstract

Label-free measurements of the reaction kinetics of a small sample volume are essential for efficient drug discovery, requiring methods and systems that are rapid, accurate, and cost-effective. Herein, we present an integrated optofluidic system for label-free characterization of reactions in a nanoliter reagent volume. This system contains a droplet-based microfluidic sampling section and an optical fiber-based spectroscopy detection section. By manipulating droplets containing reagents at certain concentrations at different times, quantifiable measurements via absorption spectroscopy can be made in a simple, sensitive, and high-throughput manner. We have demonstrated our system's capability by performing potency (IC50) assays of an inhibitor in a TEM-1 β-lactamase (enzyme) and nitrocefin (substrate) system. This integrated platform can potentially provide an automated, label-free, and low-cost method for many other assays of reaction kinetics.
 beta-Lactamases - metabolism Drug Discovery - methods Drug Evaluation, Preclinical - methods Enzyme Inhibitors - metabolism High-Throughput Screening Assays - instrumentation High-Throughput Screening Assays - methods Inhibitory Concentration 50 Kinetics Microfluidics - instrumentation Microfluidics - methods Spectrum Analysis - instrumentation Spectrum Analysis - methods

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