Lack of Sympathetic and Cholinergic Influences on Cerebral Vasodilation Caused by Sciatic Nerve Stimulation in the Rat

Setsuro Ibayashi; Al C Ngai; Matthew A Howard III; Joseph R Meno; Marc R Mayberg; H. Richard Winn

doi:10.1038/jcbfm.1991.120

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Lack of Sympathetic and Cholinergic Influences on Cerebral Vasodilation Caused by Sciatic Nerve Stimulation in the Rat

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Lack of Sympathetic and Cholinergic Influences on Cerebral Vasodilation Caused by Sciatic Nerve Stimulation in the Rat

Setsuro Ibayashi, Al C Ngai, Matthew A Howard III, Joseph R Meno, Marc R Mayberg and H. Richard Winn

Journal of cerebral blood flow and metabolism, Vol.11(4), pp.678-683

06/29/2016

DOI: 10.1038/jcbfm.1991.120

PMID: 2050756

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Abstract

We studied the influences of sympathetic and cholinergic mechanisms on pial arteriolar responses during cortical activation in the rat. Adult male Sprague-Dawley rats were anesthetized with α-chloralose and urethane and mechanically ventilated. Pial arterioles on the somatosensory cortex were visualized on a video monitor through a closed cranial window. Changes in arteriolar diameter induced by sciatic nerve stimulation (0.2 V, 5 Hz, 5 ms, for 20 s) were measured before and after (a) ipsilateral superior cervical ganglionectomy (n = 5), (b) intravenous (0.5 mg/kg) administration and topical (10−5 M) application of atropine ( n = 5), and (c) lesion of the nucleus basalis magnocellularis (the major source of intracerebral acetylcholine neurons, n = 7). Unilateral nucleus basalis magnocellularis lesions were performed stereotactically by injection of ibotenic acid (25 nmol/μl). Sensory cortex cholinergic denervation was confirmed histologically. These treatments had no significant effect on arteriolar responses to sciatic nerve stimulation. Thus, the present results suggest that neither sympathetic nor cholinergic mechanisms play a significant role in somatosensory evoked cerebral vasodilation.

Details

Title: Subtitle: Lack of Sympathetic and Cholinergic Influences on Cerebral Vasodilation Caused by Sciatic Nerve Stimulation in the Rat
Creators: Setsuro Ibayashi - Department of Neurological Surgery, University of Washington School of Medicine, Seattle, Washington, U.S.A
Al C Ngai - Department of Neurological Surgery, University of Washington School of Medicine, Seattle, Washington, U.S.A
Matthew A Howard III - Department of Neurological Surgery, University of Washington School of Medicine, Seattle, Washington, U.S.A
Joseph R Meno - Department of Neurological Surgery, University of Washington School of Medicine, Seattle, Washington, U.S.A
Marc R Mayberg - Department of Neurological Surgery, University of Washington School of Medicine, Seattle, Washington, U.S.A
H. Richard Winn - Department of Neurological Surgery, University of Washington School of Medicine, Seattle, Washington, U.S.A
Resource Type: Journal article
Publication Details: Journal of cerebral blood flow and metabolism, Vol.11(4), pp.678-683
DOI: 10.1038/jcbfm.1991.120
PMID: 2050756
ISSN: 0271-678X
eISSN: 1559-7016
Language: English
Date published: 06/29/2016
Academic Unit: Neurology; Iowa Neuroscience Institute; Neurosurgery; Otolaryngology
Record Identifier: 9984020618302771

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