Journal article
Lack of airway submucosal glands impairs respiratory host defenses
eLife, Vol.9, pp.1-25
10/07/2020
DOI: 10.7554/eLife.59653
PMCID: PMC7541087
PMID: 33026343
Abstract
Submucosal glands (SMGs) are a prominent structure that lines human cartilaginous airways. Although it has been assumed that SMGs contribute to respiratory defense, that hypothesis has gone without a direct test. Therefore, we studied pigs, which have lungs like humans, and disrupted the gene for ectodysplasin (
), which initiates SMG development.
pigs lacked SMGs throughout the airways. Their airway surface liquid had a reduced ability to kill bacteria, consistent with SMG production of antimicrobials. In wild-type pigs, SMGs secrete mucus that emerges onto the airway surface as strands. Lack of SMGs and mucus strands disrupted mucociliary transport in
pigs. Consequently,
pigs failed to eradicate a bacterial challenge in lung regions normally populated by SMGs. These in vivo and ex vivo results indicate that SMGs are required for normal antimicrobial activity and mucociliary transport, two key host defenses that protect the lung.
Details
- Title: Subtitle
- Lack of airway submucosal glands impairs respiratory host defenses
- Creators
- Lynda S Ostedgaard - University of IowaMargaret P Price - University of IowaKristin M Whitworth - University of MissouriMahmoud H Abou Alaiwa - University of IowaAnthony J Fischer - University of IowaAkshaya Warrier - University of IowaMelissa Samuel - University of MissouriLee D Spate - University of MissouriPatrick D Allen - University of IowaBrieanna M Hilkin - University of IowaGuillermo S Romano Ibarra - University of IowaMiguel E Ortiz Bezara - University of IowaBrian J Goodell - University of IowaSteven E Mather - University of IowaLinda S Powers - University of IowaMallory R Stroik - University of IowaNicholas D Gansemer - University of IowaCamilla E Hippee - University of IowaKeyan Zarei - University of IowaJ Adam Goeken - University of IowaThomas R Businga - University of IowaEric A Hoffman - University of IowaDavid K Meyerholz - University of IowaRandall S Prather - University of MissouriDavid A Stoltz - University of IowaMichael J Welsh - University of Iowa
- Resource Type
- Journal article
- Publication Details
- eLife, Vol.9, pp.1-25
- DOI
- 10.7554/eLife.59653
- PMID
- 33026343
- PMCID
- PMC7541087
- NLM abbreviation
- Elife
- ISSN
- 2050-084X
- eISSN
- 2050-084X
- Grant note
- P30 ES005605 / NIEHS NIH HHS K08 HL136927 / NHLBI NIH HHS K08 HL135433 / NHLBI NIH HHS Howard Hughes Medical Institute T32 HL007638 / NHLBI NIH HHS T32 GM007337 / NIGMS NIH HHS CFF STOLTZ16XX0 / Cystic Fibrosis Foundation P01 HL091842 / NHLBI NIH HHS S10 OD018526 / NIH HHS R01 HL136813 / NHLBI NIH HHS CFF FISCHE16I0 / Cystic Fibrosis Foundation P01 HL051670 / NHLBI NIH HHS P30 DK054759 / NIDDK NIH HHS
- Language
- English
- Date published
- 10/07/2020
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Neurology; Radiology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Pulmonary Medicine; Stead Family Department of Pediatrics; Pathology; Fraternal Order of Eagles Diabetes Research Center; Neurosurgery; Internal Medicine
- Record Identifier
- 9984186566802771
Metrics
16 Record Views