Lack of carbon air filtration impacts early embryo development

Erika M Munch; Amy E Sparks; Hakan E Duran; Bradley J Van Voorhis

doi:10.1007/s10815-015-0495-1

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Lack of carbon air filtration impacts early embryo development

Journal article

Open access

Peer reviewed

Lack of carbon air filtration impacts early embryo development

Erika M Munch, Amy E Sparks, Hakan E Duran and Bradley J Van Voorhis

Journal of assisted reproduction and genetics, Vol.32(7), pp.1009-1017

07/2015

DOI: 10.1007/s10815-015-0495-1

PMCID: PMC4531868

PMID: 26003657

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https://doi.org/10.1007/s10815-015-0495-1View

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Abstract

To assess human fertilization and preimplantation embryo development in the presence and in the absence of carbon filtration This is a retrospective cohort analysis of fresh, controlled ovarian hyperstimulation cycles as well as previously cryopreserved pronuclear stage embryo transfer cycles in a single IVF center. Embryo development and cycle-based outcomes were compared among three groups: 1) when carbon filtration was present, 2) when carbon filtration was absent, and 3) when carbon filtration had been restored. A total of 524 fresh cycles and 156 cryopreserved embryo cycles were analyzed. Fertilization, cleavage, and blastocyst conversion rates for fresh cycles all declined during the period of absent carbon filtration and recovered after the restoration of carbon filtration. Cryopreserved embryos that were thawed and cultured during the period of absent filtration did not have changes in cleavage or blastocyst conversion rates compared to periods where carbon filtration was present. Clinical pregnancy and live birth rates were unchanged among the three time periods. The absence of carbon filtration in an IVF laboratory air handler is associated with poor fertilization and early embryo development for fresh cycles. Because development of previously frozen pronuclear stage embryos was unaffected, the lack of carbon filtration may preferentially affect embryos in the peri-fertilization period. Carbon filtration is an integral part to a successful human in-vitro fertilization laboratory.

Laboratories - standards

Filtration

Ovulation Induction

Humans

Blastocyst - cytology

Blastocyst - physiology

Male

Embryo Culture Techniques

Embryonic Development

Carbon

Cryopreservation - methods

Pregnancy

Fertilization in Vitro

Adult

Female

Retrospective Studies

Air Pollution, Indoor - adverse effects

Infant, Newborn

Pregnancy Outcome

Pregnancy Rate

Cohort Studies

Details

Title: Subtitle: Lack of carbon air filtration impacts early embryo development
Creators: Erika M Munch - Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA
Amy E Sparks
Hakan E Duran
Bradley J Van Voorhis
Resource Type: Journal article
Publication Details: Journal of assisted reproduction and genetics, Vol.32(7), pp.1009-1017
DOI: 10.1007/s10815-015-0495-1
PMID: 26003657
PMCID: PMC4531868
NLM abbreviation: J Assist Reprod Genet
ISSN: 1058-0468
eISSN: 1573-7330
Publisher: Netherlands
Language: English
Date published: 07/2015
Academic Unit: Obstetrics and Gynecology
Record Identifier: 9983930957802771

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