Journal article
Lactate dehydrogenase isoform activity mapping in patients with intra-amniotic infection
American journal of obstetrics and gynecology, Vol.195(4), pp.1045-1052
10/01/2006
DOI: 10.1016/j.ajog.2006.05.048
PMID: 16875649
Abstract
Five distinct lactate dehydrogenase isoenzymes have been described. We sought to illustrate the specific amniotic fluid lactate dehydrogenase isoenzyme activity profiles in women with intra-amniotic infection.
Amniotic fluid was retrieved from 82 women who were stratified in the following groups: (1) positive amniotic fluid cultures (n = 23 women; gestational age, 26 weeks [range, 21-32 weeks]); (2) negative amniotic fluid cultures (n = 22 women; gestational age, 30 weeks [range, 16-36 weeks]); (3) second trimester control (normal genetic karyotype; n = 17 women; gestational age, 18 weeks [range, 16-22 weeks]); and (4) third trimester control (fetal lung maturity testing; n = 20 women; gestational age, 36 weeks [range, 31-38 weeks]). The optical density of each isoform was determined relative to a standard with 5 known lactate dehydrogenase isoenzyme activities. Total lactate dehydrogenase activity was measured by the clinical laboratory immediately after retrieval and by a kinetic UV spectrophotometric assay at the time of the isoelectric focusing.
Infection increased total lactate dehydrogenase activity: positive amniotic fluid cultures (median, 762.4 [range, 169.3-3374.8]) vs negative amniotic fluid cultures (median, 203.7 [range, 57.8-1939.3]; U/L;
P < .001]). Lactate dehydrogenase isoform profiling identified significant and specific increases in lactate dehydrogenase isoforms 3, 4 (
P < .01), and 5 (
P < .05) in positive amniotic fluid cultures compared to the negative amniotic fluid cultures group. A selective up-regulation in lactate dehydrogenase isoform 5 was identified at term in healthy subjects.
Intra-amniotic infection is characterized by an increase in the activities of lactate dehydrogenase isoforms 3, 4, and 5; advancing gestational age demonstrates an up-regulation of isoform 5 only.
Details
- Title: Subtitle
- Lactate dehydrogenase isoform activity mapping in patients with intra-amniotic infection
- Creators
- Lissa K. Magloire - Yale UniversityCatalin S. Buhimschi - Department of Obstetrics, Gynecology, and Reproductive SciencesChristian M. Pettker - Department of Obstetrics, Gynecology, and Reproductive SciencesAnna K. Sfakianaki - Department of Obstetrics, Gynecology, and Reproductive SciencesBenjamin D. Hamar - Department of Obstetrics, Gynecology, and Reproductive SciencesVineet Bhandari - Yale UniversityIrina A. Buhimschi - Department of Obstetrics, Gynecology, and Reproductive Sciences
- Resource Type
- Journal article
- Publication Details
- American journal of obstetrics and gynecology, Vol.195(4), pp.1045-1052
- DOI
- 10.1016/j.ajog.2006.05.048
- PMID
- 16875649
- NLM abbreviation
- Am J Obstet Gynecol
- ISSN
- 0002-9378
- eISSN
- 1097-6868
- Publisher
- Mosby, Inc
- Number of pages
- 8
- Language
- English
- Date published
- 10/01/2006
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9985244555702771
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