Laminin and growth factor receptor activation stimulates differential growth responses in subpopulations of adult DRG neurons

Budd A Tucker; Masuma Rahimtula; Karen M Mearow

doi:10.1111/j.1460-9568.2006.04963.x

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Laminin and growth factor receptor activation stimulates differential growth responses in subpopulations of adult DRG neurons

Journal article

Peer reviewed

Laminin and growth factor receptor activation stimulates differential growth responses in subpopulations of adult DRG neurons

Budd A Tucker, Masuma Rahimtula and Karen M Mearow

The European journal of neuroscience, Vol.24(3), pp.676-690

08/2006

DOI: 10.1111/j.1460-9568.2006.04963.x

PMID: 16930399

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Abstract

Neurons in the adult rat dorsal root ganglion (DRG) can be classified into at least three separate subpopulations based on morphologic and phenotypic differences. In this study we have focused on the growth response of these specific subpopulations in vitro with respect to laminin (LN) and growth factor receptor activation. Using a cell selection approach we show that LN-induced neurite growth occurs in the absence of added trophic factors only in heavy-chain neurofilament-positive and calcitonin gene-related peptide-positive DRG neurons [nerve growth factor (NGF)-responsive population]. In contrast, LN alone is not sufficient to stimulate significant neurite growth from lectin Griffonia simplicifolia IB4-positive neurons (IB4+ve), although it is still required to elicit a growth response from these cells in the presence of glial-derived neurotrophic factor (GDNF, e.g. neurite growth occurred only when cells were plated on LN in the presence of GDNF). By using chemical inhibitors we demonstrate that only the phosphatidylinositol 3 kinase (PI 3-K)/Akt pathway is required for neurite growth from the NGF-responsive cell population. However, both the PI 3-K/Akt and MEK/mitogen-activated protein kinase signaling pathways are required for neurite growth from the IB4+ve cell population. Thus, we have identified specific signaling events and environmental requirements associated with neurite growth for different subpopulations of adult DRG neurons, pointing to potential therapeutic targets while identifying an inability for any one treatment alone to repair peripheral nerve damage.

Immunohistochemistry

Laminin - pharmacology

MAP Kinase Signaling System - physiology

Nerve Regeneration - physiology

Ganglia, Spinal - cytology

Phosphatidylinositol 3-Kinases - metabolism

Phosphatidylinositol 3-Kinases - antagonists & inhibitors

Glial Cell Line-Derived Neurotrophic Factor - metabolism

Neurites - ultrastructure

Glial Cell Line-Derived Neurotrophic Factor - pharmacology

Calcitonin Gene-Related Peptide - metabolism

Proto-Oncogene Proteins c-akt - metabolism

Cell Differentiation - physiology

Neurites - drug effects

Neurons, Afferent - drug effects

Neurons, Afferent - cytology

Neurofilament Proteins - drug effects

Cells, Cultured

Enzyme Inhibitors - pharmacology

Rats

Neurites - metabolism

Rats, Sprague-Dawley

Receptors, Growth Factor - agonists

Animals

Growth Cones - metabolism

MAP Kinase Signaling System - drug effects

Growth Cones - ultrastructure

Cell Differentiation - drug effects

Nerve Regeneration - drug effects

Growth Cones - drug effects

Laminin - metabolism

Neurons, Afferent - metabolism

Ganglia, Spinal - drug effects

Neurofilament Proteins - metabolism

Plant Lectins - metabolism

Proto-Oncogene Proteins c-akt - antagonists & inhibitors

Ganglia, Spinal - metabolism

Receptors, Growth Factor - metabolism

Details

Title: Subtitle: Laminin and growth factor receptor activation stimulates differential growth responses in subpopulations of adult DRG neurons
Creators: Budd A Tucker - Division of Basic Medical Sciences, Memorial University of Newfoundland, St. John's, NL, Canada A1B 3V6
Masuma Rahimtula
Karen M Mearow
Resource Type: Journal article
Publication Details: The European journal of neuroscience, Vol.24(3), pp.676-690
Publisher: France
DOI: 10.1111/j.1460-9568.2006.04963.x
PMID: 16930399
ISSN: 0953-816X
eISSN: 1460-9568
Language: English
Date published: 08/2006
Academic Unit: Ophthalmology and Visual Sciences
Record Identifier: 9983979921602771

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