Large inverted repeats in the vicinity of a single double-strand break strongly affect repair in yeast diploids lacking Rad51

Brandon Downing; Rachel Morgan; Kelly VanHulle; Angela Deem; Anna Malkova

doi:10.1016/j.mrfmmm.2008.07.013

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Journal article

Large inverted repeats in the vicinity of a single double-strand break strongly affect repair in yeast diploids lacking Rad51

Brandon Downing, Rachel Morgan, Kelly VanHulle, Angela Deem and Anna Malkova

Mutation research, Vol.645(1-2), pp.9-18

10/14/2008

DOI: 10.1016/j.mrfmmm.2008.07.013

PMCID: PMC2643024

PMID: 18755201

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Abstract

DNA double-strand breaks (DSBs) are critical lesions that can lead to cell death or chromosomal rearrangements. Rad51 is necessary for most mitotic and meiotic DSB repair events, although a number of RAD51-independent pathways exist. Previously, we described DSB repair in rad51Delta yeast diploids that was stimulated by a DNA region termed "facilitator of break-induced replication" (FBI) located approximately 30kb from the site of an HO-induced DSB. Here, we demonstrate that FBI is a large inverted DNA repeat that channels the repair of DSBs into the single-strand annealing-gross chromosomal rearrangements (SSA-GCR) pathway. Further, analysis of DSB repair in rad54Delta cells allowed us to propose that the SSA-GCR repair pathway is suppressed in the presence of Rad51p. Therefore, an additional role of Rad51 might be to protect eukaryotic genomes from instabilities by preventing chromosomal rearrangements.

Base Sequence

Chromosomes, Fungal - genetics

Diploidy

DNA Breaks, Double-Stranded

DNA Helicases

DNA Primers - genetics

DNA Repair - genetics

DNA Repair Enzymes

DNA, Fungal - genetics

DNA, Fungal - metabolism

Gene Conversion

Genes, Fungal

Genomic Instability

Inverted Repeat Sequences

Rad51 Recombinase - genetics

Rad51 Recombinase - metabolism

Saccharomyces cerevisiae - genetics

Saccharomyces cerevisiae - metabolism

Saccharomyces cerevisiae Proteins - genetics

Saccharomyces cerevisiae Proteins - metabolism

Symporters - genetics

Details

Title: Subtitle: Large inverted repeats in the vicinity of a single double-strand break strongly affect repair in yeast diploids lacking Rad51
Creators: Brandon Downing - Department of Biology, School of Science, IUPUI, Indianapolis, IN 46202-5132, USA
Rachel Morgan
Kelly VanHulle
Angela Deem
Anna Malkova
Resource Type: Journal article
Publication Details: Mutation research, Vol.645(1-2), pp.9-18
DOI: 10.1016/j.mrfmmm.2008.07.013
PMID: 18755201
PMCID: PMC2643024
NLM abbreviation: Mutat Res
ISSN: 0027-5107
eISSN: 1873-135X
Grant note: GM20056 / NIGMS NIH HHS R15 GM074657 / NIGMS NIH HHS 1R15GM074657 / NIGMS NIH HHS 3R15GM074657-01A1S1 / NIGMS NIH HHS R01 GM020056 / NIGMS NIH HHS R01 GM084242 / NIGMS NIH HHS R37 GM020056 / NIGMS NIH HHS R15 GM074657-01A1S1 / NIGMS NIH HHS R15 GM074657-01A1 / NIGMS NIH HHS
Language: English
Date published: 10/14/2008
Academic Unit: Biology
Record Identifier: 9984217535102771

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