Journal article
Large-scale gene discovery in human airway epithelia reveals novel transcripts
Physiological genomics, Vol.17(1), pp.69-77
03/12/2004
DOI: 10.1152/physiolgenomics.00188.2003
PMID: 14701920
Abstract
The airway epithelium represents an important barrier between the host and the environment. It is a first site of contact with pathogens, particulates, and other stimuli, and has evolved the means to dynamically respond to these challenges. In an effort to define the transcript profile of airway epithelia, we created and sequenced cDNA libraries from cystic fibrosis (CF) and non-CF epithelia and from human lung tissue. Sequencing of these libraries produced approximately 53,000 3'-expressed sequence tags (3'-ESTs). From these, a nonredundant UniGene set of more than 19,000 sequences was generated. Despite the relatively small contribution of airway epithelia to the total mass of the lung, focused gene discovery in this tissue yielded novel results. The ESTs included several thousand transcripts (6,416) not previously identified from cDNA sequences as expressed in the lung. Among the abundant transcripts were several genes involved in host defense. Most importantly, the set also included 879 3'-ESTs that appear to be novel sequences not previously represented in the National Center for Biotechnology Information UniGene collection. This UniGene set should be useful for studies of pulmonary diseases involving the airway epithelium including cystic fibrosis, respiratory infections and asthma. It also provides a reagent for large-scale expression profiling.
Details
- Title: Subtitle
- Large-scale gene discovery in human airway epithelia reveals novel transcripts
- Creators
- Todd E Scheetz - Department of Pediatrics, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USAJoseph ZabnerMichael J WelshJustin CocoMari de Fatima EyestoneMaria BonaldoTamara KucabaThomas L CasavantM Bento SoaresPaul B McCray Jr
- Resource Type
- Journal article
- Publication Details
- Physiological genomics, Vol.17(1), pp.69-77
- DOI
- 10.1152/physiolgenomics.00188.2003
- PMID
- 14701920
- NLM abbreviation
- Physiol Genomics
- ISSN
- 1531-2267
- eISSN
- 1531-2267
- Publisher
- United States
- Grant note
- HL-51670 / NHLBI NIH HHS HL-61234 / NHLBI NIH HHS P30-DK-54759 / NIDDK NIH HHS
- Language
- English
- Date published
- 03/12/2004
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Neurology; Electrical and Computer Engineering; Molecular Physiology and Biophysics; Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Neurosurgery; Internal Medicine; Ophthalmology and Visual Sciences
- Record Identifier
- 9983980074402771
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