Journal article
Laser fluorescence bronchoscopy for detection of fluorescent reporter genes in airway epithelia
Gene therapy, Vol.9(23), pp.1639-1644
12/2002
DOI: 10.1038/sj.gt.3301825
PMID: 12424617
Abstract
Current methods for detecting successful gene transfer to airway epithelia involve obtaining a sample of the target tissue. This may affect the longevity of expression of the transgene under evaluation. We describe a laser fluorescence bronchoscopic system that can detect the expression of the fluorescent protein, green fluorescence protein (GFP), in the airway of monkeys that have been transfected with adenovirus, without the need for obtaining tissue. This technique will have applications in pre-clinical and clinical studies of gene transfer to airway epithelia and other surface epithelia accessible by endoscopy.
Details
- Title: Subtitle
- Laser fluorescence bronchoscopy for detection of fluorescent reporter genes in airway epithelia
- Creators
- C P Rooney - University of IowaM Suter - University of IowaG McLennan - University of IowaM Donnelley - University of IowaJ Reinhardt - University of IowaA Delsing - University of IowaE A Hoffman - University of IowaJ Zabner - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Gene therapy, Vol.9(23), pp.1639-1644
- DOI
- 10.1038/sj.gt.3301825
- PMID
- 12424617
- NLM abbreviation
- Gene Ther
- ISSN
- 0969-7128
- eISSN
- 1476-5462
- Grant note
- DK-54759 / NIDDK NIH HHS
- Language
- English
- Date published
- 12/2002
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
- Record Identifier
- 9984197151602771
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