Laser-induced mouse model of chronic ocular hypertension

Sinisa D Grozdanic; Daniel M Betts; Donald S Sakaguchi; Rachell A Allbaugh; Young H Kwon; Randy H Kardon

doi:10.1167/iovs.03-0015

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Laser-induced mouse model of chronic ocular hypertension

Journal article

Peer reviewed

Laser-induced mouse model of chronic ocular hypertension

Sinisa D Grozdanic, Daniel M Betts, Donald S Sakaguchi, Rachell A Allbaugh, Young H Kwon and Randy H Kardon

Investigative ophthalmology & visual science, Vol.44(10), pp.4337-4346

10/2003

DOI: 10.1167/iovs.03-0015

PMID: 14507878

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Abstract

To develop an inducible mouse model of glaucoma. An obstruction of aqueous humor outflow in adult C57BL6/J mice was induced by combined injection of indocyanine green (ICG) dye into the anterior chamber and diode laser treatment. To evaluate intraocular pressure (IOP), tonometry was performed with a modified Goldmann tonometer. The function of the retina was evaluated with electroretinography (ERG). IOP was significantly elevated in surgical eyes compared with control eyes: before surgery, 15.2 +/- 0.6 mm Hg; 10 days after surgery, 33.6 +/- 1.5 mm Hg (P < 0.001); and 30 days after surgery, 27.4 +/- 1.2 mm Hg (P < 0.001). However, 60 days after surgery, IOP in the surgical eyes decreased to 19.5 +/- 0.9 mm Hg and was not significantly different compared with control eyes (control, 17.3 +/- 0.7 mm Hg; P = 0.053). ERG amplitudes, expressed as a ratio (surgical/control), were decreased in surgical eyes. The amplitudes for b-wave were: before surgery, 107.6% +/- 4.6%; 28 days after surgery, 61% +/- 4% (P < 0.001); and 56 days after surgery, 62% +/- 5.6% (P < 0.001). Oscillatory potentials were the most dramatically affected: before surgery, 108.6% +/- 6.7%; 28 days after surgery, 57.5% +/- 5% (P < 0.01); and 56 days after surgery, 57% +/- 8.5% (P < 0.001). Amplitudes of the a-waves had relatively smaller but still significant deficits: before surgery, 105.8% +/- 6.9%; 28 days after surgery, 72.2% +/- 5.4% (P < 0.01); and 56 days after surgery, 79.8% +/- 11.0% (P < 0.01). Histologic analysis of the surgical eyes revealed development of anterior synechia, loss of retinal ganglion cells (RGCs), and thinning of all retinal layers. Electron microscopy of optic nerve cross sections revealed swelling and degeneration of the large diameter axons and gliosis. Diode laser treatment of ICG saturated episcleral veins causes a chronic elevation of IOP and sustained ERG deficits.

Electroretinography

Trabecular Meshwork - pathology

Optic Nerve - pathology

Intraocular Pressure

Ocular Hypertension - pathology

Laser Therapy

Mice, Inbred C57BL

Indocyanine Green

Ocular Hypertension - metabolism

Fluorophotometry

Trabecular Meshwork - surgery

Retinal Ganglion Cells - pathology

Aqueous Humor - metabolism

Animals

Trabecular Meshwork - metabolism

Tonometry, Ocular

Mice

Chronic Disease

Oscillometry

Disease Models, Animal

Details

Title: Subtitle: Laser-induced mouse model of chronic ocular hypertension
Creators: Sinisa D Grozdanic - College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA. sgrozdan@iastate.edu
Daniel M Betts
Donald S Sakaguchi
Rachell A Allbaugh
Young H Kwon
Randy H Kardon
Resource Type: Journal article
Publication Details: Investigative ophthalmology & visual science, Vol.44(10), pp.4337-4346
DOI: 10.1167/iovs.03-0015
PMID: 14507878
NLM abbreviation: Invest Ophthalmol Vis Sci
ISSN: 0146-0404
eISSN: 1552-5783
Publisher: United States
Language: English
Date published: 10/2003
Academic Unit: Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9983980024302771

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