Latent and viral replicative transcription in vivo from the BamHI K fragment of Epstein-Barr virus DNA

R Weigel; G Miller

doi:10.1128/jvi.54.2.501-508.1985

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Latent and viral replicative transcription in vivo from the BamHI K fragment of Epstein-Barr virus DNA

Journal article

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Latent and viral replicative transcription in vivo from the BamHI K fragment of Epstein-Barr virus DNA

R Weigel and G Miller

Journal of virology, Vol.54(2), pp.501-508

1985

DOI: 10.1128/jvi.54.2.501-508.1985

PMCID: PMC254822

PMID: 2985812

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Abstract

We mapped one latent and two replicative messages transcribed in vivo from the BamHI K fragment of the Epstein-Barr virus genome. The exon encoding Epstein-Barr nuclear antigen (EBNA), a major latent product, is 2,028 bases; the 3' end of this exon occurs 30 bases after the polyadenylation signal AATAAA, and the 5' end occurs within a splice acceptor site. The open reading frame which encodes the EBNA peptide is completely contained within this coding exon. The exon was faithfully transcribed after transfection of cloned BamHI-K into either COS-1 or TK- mouse L cells. In lymphocytes the abundance of the EBNA message is increased after cycloheximide treatment. The two viral replicative genes completely contained in BamHI-K were not transcribed in line X50-7, in which the genome is tightly latent. In contrast to the EBNA message, these mRNAs of 1.3 and 2.1 kilobases are inducible with phorbol ester and are unspliced. Their promoter regions are similar to those of each other and to replicative promoters mapped in other regions of the Epstein-Barr virus genome. An unusual feature of these replicative genes is that the smaller mRNA begins within a long open reading frame of the larger mRNA. The identification of the structure of latent and replicative genes within one DNA fragment will facilitate analysis of regulation of expression for the two life cycles of the virus.

Microbiology

Virology

Biological and medical sciences

Fundamental and applied biological sciences. Psychology

Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains

Details

Title: Subtitle: Latent and viral replicative transcription in vivo from the BamHI K fragment of Epstein-Barr virus DNA
Creators: R Weigel - Yale University
G Miller - Yale University
Resource Type: Journal article
Publication Details: Journal of virology, Vol.54(2), pp.501-508
DOI: 10.1128/jvi.54.2.501-508.1985
PMID: 2985812
PMCID: PMC254822
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: American Society for Microbiology
Language: English
Date published: 1985
Academic Unit: Molecular Physiology and Biophysics; Anatomy and Cell Biology; Surgery; Biochemistry and Molecular Biology
Record Identifier: 9984284338302771

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