LeCTR1, a Tomato CTR1-Like Gene, Demonstrates Ethylene Signaling Ability in Arabidopsis and Novel Expression Patterns in Tomato

Julie Leclercq; Lori C Adams-Phillips; Hicham Zegzouti; Brian Jones; Alain Latché; James J Giovannoni; Jean-Claude Pech; Mondher Bouzayen

doi:10.1104/pp.009415

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LeCTR1, a Tomato CTR1-Like Gene, Demonstrates Ethylene Signaling Ability in Arabidopsis and Novel Expression Patterns in Tomato

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LeCTR1, a Tomato CTR1-Like Gene, Demonstrates Ethylene Signaling Ability in Arabidopsis and Novel Expression Patterns in Tomato

Julie Leclercq, Lori C Adams-Phillips, Hicham Zegzouti, Brian Jones, Alain Latché, James J Giovannoni, Jean-Claude Pech and Mondher Bouzayen

Plant physiology (Bethesda), Vol.130(3), pp.1132-1142

11/01/2002

DOI: 10.1104/pp.009415

PMCID: PMC166634

PMID: 12427980

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Abstract

Abstract LeCTR1 was initially isolated by both differential display reverse transcriptase-polymerase chain reaction screening for tomato (Lycopersicon esculentum) fruit ethylene-inducible genes and through homology with the Arabidopsis CTR1 cDNA. LeCTR1 shares strong nucleotide sequence homology with Arabidopsis CTR1, a gene acting downstream of the ethylene receptor and showing similarity to the Raf family of serine/threonine protein kinases. The length of the LeCTR1 transcribed region from ATG to stop codon (12,000 bp) is more than twice that of Arabidopsis CTR1(4,700 bp). Structural analysis reveals perfect conservation of both the number and position of introns and exons in LeCTR1and Arabidopsis CTR1. The introns inLeCTR1 are much longer, however. To address whether this structural conservation is indicative of functional conservation of the corresponding proteins, we expressed LeCTR1 in the Arabidopsis ctr1-1 (constitutive triple response 1) mutant under the direction of the 35S promoter. Our data clearly show that ectopic expression of LeCTR1 in the Arabidopsis ctr1-1 mutant can restore normal ethylene signaling. The recovery of normal ethylene sensitivity upon heterologous expression of LeCTR1 was also confirmed by restored glucose sensitivity absent in the Arabidopsisctr1-1 mutant. Expression studies confirm ethylene responsiveness of LeCTR1 in various tissues, including ripening fruit, and may suggest the evolution of alternate regulatory mechanisms in tomato versus Arabidopsis.

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Title: Subtitle: LeCTR1, a Tomato CTR1-Like Gene, Demonstrates Ethylene Signaling Ability in Arabidopsis and Novel Expression Patterns in Tomato
Creators: Julie Leclercq - Unité Mixte de Recherche 990, Institut National de la Recherche Agronomique/Institut National Polytechnique-Ecole Nationale Supérieure Agronomique, Boite Postale 107 Auzeville, 31326 Castanet Tolosan cedex, France (J.L., H.Z., B.J., A.L., J.-C.P., M.B.); and
Lori C Adams-Phillips - United States Department of Agriculture-Agricultural Research Service and Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, New York 14853 (L.C.A.-P., J.J.G.)
Hicham Zegzouti - Unité Mixte de Recherche 990, Institut National de la Recherche Agronomique/Institut National Polytechnique-Ecole Nationale Supérieure Agronomique, Boite Postale 107 Auzeville, 31326 Castanet Tolosan cedex, France (J.L., H.Z., B.J., A.L., J.-C.P., M.B.); and
Brian Jones - Unité Mixte de Recherche 990, Institut National de la Recherche Agronomique/Institut National Polytechnique-Ecole Nationale Supérieure Agronomique, Boite Postale 107 Auzeville, 31326 Castanet Tolosan cedex, France (J.L., H.Z., B.J., A.L., J.-C.P., M.B.); and
Alain Latché - Unité Mixte de Recherche 990, Institut National de la Recherche Agronomique/Institut National Polytechnique-Ecole Nationale Supérieure Agronomique, Boite Postale 107 Auzeville, 31326 Castanet Tolosan cedex, France (J.L., H.Z., B.J., A.L., J.-C.P., M.B.); and
James J Giovannoni - United States Department of Agriculture-Agricultural Research Service and Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, New York 14853 (L.C.A.-P., J.J.G.)
Jean-Claude Pech - Unité Mixte de Recherche 990, Institut National de la Recherche Agronomique/Institut National Polytechnique-Ecole Nationale Supérieure Agronomique, Boite Postale 107 Auzeville, 31326 Castanet Tolosan cedex, France (J.L., H.Z., B.J., A.L., J.-C.P., M.B.); and
Mondher Bouzayen - Unité Mixte de Recherche 990, Institut National de la Recherche Agronomique/Institut National Polytechnique-Ecole Nationale Supérieure Agronomique, Boite Postale 107 Auzeville, 31326 Castanet Tolosan cedex, France (J.L., H.Z., B.J., A.L., J.-C.P., M.B.); and
Resource Type: Journal article
Publication Details: Plant physiology (Bethesda), Vol.130(3), pp.1132-1142
DOI: 10.1104/pp.009415
PMID: 12427980
PMCID: PMC166634
NLM abbreviation: Plant Physiol
ISSN: 0032-0889
eISSN: 1532-2548
Language: English
Date published: 11/01/2002
Academic Unit: Biology
Record Identifier: 9984217534602771

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