Logo image
Lectin-oral streptococci interactions
Journal article   Open access   Peer reviewed

Lectin-oral streptococci interactions

Whingming Lee, AMC De La Barca, D Drake and R Doyle
Journal of medical microbiology, Vol.47(1), pp.29-37
01/01/1998
DOI: 10.1099/00222615-47-1-29
PMID: 9449947
url
https://doi.org/10.1099/00222615-47-1-29View
Published (Version of record) Open Access

Abstract

Lectins of various specificities were examined for interaction with strains of oral streptococci of various species. The lectins were capable of binding galactose, N-acetylgalactosamine, glucose, N-acetylglucosamine, mannose, fucose and sialic acid. Lectin reactivities were strain-dependent in that some members of a species, but not others, could be aggregated by certain lectins. Proteolysis and extraction with hot water, guanidine hydrochloride and sodium dodecyl sulphate tended to increase the reactivity of the streptococci with lectins but did not change the recognition patterns of the bacteria. Sonication, in contrast, tended to reduce the ability of streptococci to be agglutinated by lectins. Furthermore, lectin reactivities were dependent on the growth conditions, as evidenced by changes in lectin titres following streptococcal growth in sub-inhibitory concentrations of citrate, fluoride or antibiotics. It is likely that lectins could be useful tools for epidemiological studies and to probe strain-dependent and growth-dependent surface characteristics of viridans streptococci.

Details

Metrics

Logo image