Journal article
Lef1 transcription factor expression defines airway progenitor cell targets for in utero gene therapy of submucosal gland in cystic fibrosis
American journal of respiratory cell and molecular biology, Vol.18(6), pp.750-758
06/1998
DOI: 10.1165/ajrcmb.18.6.2987
PMID: 9618379
Abstract
Cystic fibrosis (CF) has emerged as a paradigm disorder for assessing the utility of gene therapy in the treatment of genetic diseases. It is hypothesized that submucosal glands may play an important role in the pathophysiology of CF lung disease. However, this region poses several significant obstacles for gene therapy due to its inaccessibility from the lumen of adult proximal airways. In utero gene therapy to correct submucosal gland dysfunction in CF provides an attractive alternative strategy to target gland progenitor cells prior to gland formation and morphogenesis. Such approaches will require the use of integrating vectors capable of transducing expanding stem-cell/progenitor-cell populations in the lung. We described a newborn-ferret model of the proximal airway which was used to evaluate the phenotypic characteristics of submucosal gland progenitors and to test gene therapy strategies for targeting these cell types. To this end, we have isolated the ferret cDNA to the lymphoid enhancing factor 1 (Lef1) and have demonstrated that its mRNA expression specifically defines a subset of surface airway epithelial progenitor cells involved in the formation of primordial submucosal gland buds and subsequent gland morphogenesis. Such findings suggest that the transcriptional switch regulating activation of Lef1 expression defines the phenotype of early submucosal gland progenitor cells. In an effort to prove the principle of gene targeting to this progenitor-cell population, we evaluated the efficiency of recombinant retroviral vectors to target submucosal glands in a xenograft model system. Findings from these studies demonstrated successful gene targeting to progenitor cells of submucosal gland buds which was stable throughout subsequent gland development. In summary, these studies have provided evidence for the existence of phenotypically distinct submucosal gland progenitor cells which represent appropriate targets for gene therapy of submucosal glands in CF.
Details
- Title: Subtitle
- Lef1 transcription factor expression defines airway progenitor cell targets for in utero gene therapy of submucosal gland in cystic fibrosis
- Creators
- Dongsheng Duan - Department of Anatomy and Cell Biology, University of Iowa, Iowa City, Iowa 52242-1109, USAAnil SehgalJie YaoJ F Engelhardt
- Resource Type
- Journal article
- Publication Details
- American journal of respiratory cell and molecular biology, Vol.18(6), pp.750-758
- Publisher
- United States
- DOI
- 10.1165/ajrcmb.18.6.2987
- PMID
- 9618379
- ISSN
- 1044-1549
- eISSN
- 1535-4989
- Grant note
- R01 DK047967 / NIDDK NIH HHS P30 DK054759 / NIDDK NIH HHS 2RO1 47967 / PHS HHS
- Language
- English
- Date published
- 06/1998
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025315202771
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