Journal article
Leishmania chagasi: Homogenous metacyclic promastigotes isolated by buoyant density are highly virulent in a mouse model
Experimental parasitology, Vol.118(1), pp.129-133
2008
DOI: 10.1016/j.exppara.2007.06.012
PMCID: PMC2274777
PMID: 17706646
Abstract
Homogenous metacyclic promastigotes of
Leishmania chagasi were isolated by buoyant density from
in vitro heterogeneous cultures and used for biochemical characterization of isoforms of the major surface protease (MSP). Compared to stationary phase promastigotes, metacyclic cells had three times more MSP, produced 3-fold higher parasite loads in a mouse model
in vivo, and were more resistant to complement-mediated lysis
in vitro. These metacyclic
L. chagasi expressed both the virulence-associated 59-kDa, and the constitutively expressed 63-kDa, isoforms of MSP.
Details
- Title: Subtitle
- Leishmania chagasi: Homogenous metacyclic promastigotes isolated by buoyant density are highly virulent in a mouse model
- Creators
- Chaoqun Yao - Department of Internal Medicine, University of Iowa, IA 52242, USAYani Chen - Department of Internal Medicine, University of Iowa, IA 52242, USABayan Sudan - Department of Internal Medicine, University of Iowa, IA 52242, USAJohn E Donelson - Department of Biochemistry, University of Iowa, IA 52242, USAMary E Wilson - Department of Internal Medicine, University of Iowa, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Experimental parasitology, Vol.118(1), pp.129-133
- DOI
- 10.1016/j.exppara.2007.06.012
- PMID
- 17706646
- PMCID
- PMC2274777
- NLM abbreviation
- Exp Parasitol
- ISSN
- 0014-4894
- eISSN
- 1090-2449
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2008
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984002399802771
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