Journal article
Leishmania chagasi T-cell antigens identified through a double library screen
Infection and immunity, Vol.74(12), pp.6940-6948
12/2006
DOI: 10.1128/iai.02032-05
PMCID: PMC1698060
PMID: 17000724
Abstract
Control of human visceral leishmaniasis in regions where it is endemic is hampered in part by limited accessibility to medical care and emerging drug resistance. There is no available protective vaccine. Leishmania spp. protozoa express multiple antigens recognized by the vertebrate immune system. Since there is not one immunodominant epitope recognized by most hosts, strategies must be developed to optimize selection of antigens for prevention and immunodiagnosis. For this reason, we generated a cDNA library from the intracellular amastigote form of Leishmania chagasi, the cause of South American visceral leishmaniasis. We employed a two-step expression screen of the library to systematically identify T-cell antigens and T-dependent B-cell antigens. The first step was aimed at identifying the largest possible number of clones producing an epitope-containing polypeptide by screening with a pool of sera from Brazilians with documented visceral leishmaniasis. After removal of clones encoding heat shock proteins, positive clones underwent a second-step screen for their ability to cause proliferation and gamma interferon responses in T cells from immune mice. Six unique clones were selected from the second screen for further analysis. The corresponding antigens were derived from glutamine synthetase, a transitional endoplasmic reticulum ATPase, elongation factor 1gamma, kinesin K39, repetitive protein A2, and a hypothetical conserved protein. Humans naturally infected with L. chagasi mounted both cellular and antibody responses to these proteins. Preparations containing multiple antigens may be optimal for immunodiagnosis and protective vaccines.
Details
- Title: Subtitle
- Leishmania chagasi T-cell antigens identified through a double library screen
- Creators
- Daniella R A Martins - Department of Internal Medicine, University of Iowa, SW34-GH, 200 Hawkins Drive, Iowa City, IA 52242, USASelma M B JeronimoJohn E DonelsonMary E Wilson
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.74(12), pp.6940-6948
- DOI
- 10.1128/iai.02032-05
- PMID
- 17000724
- PMCID
- PMC1698060
- NLM abbreviation
- Infect Immun
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Publisher
- United States
- Grant note
- AI 067874 / NIAID NIH HHS TW 01369 / FIC NIH HHS AI 32135 / NIAID NIH HHS TW 00007 / FIC NIH HHS AI 045540 / NIAID NIH HHS AI 048822 / NIAID NIH HHS R03 TW001369 / FIC NIH HHS R01 AI048822 / NIAID NIH HHS R21 AI032135 / NIAID NIH HHS R01 AI045540 / NIAID NIH HHS R01 AI032135 / NIAID NIH HHS AI 059451 / NIAID NIH HHS R01 AI059451 / NIAID NIH HHS R01 AI067874 / NIAID NIH HHS P50 AI030639 / NIAID NIH HHS
- Language
- English
- Date published
- 12/2006
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984002396802771
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