Leishmania chagasi: The α-tubulin intercoding region results in constant levels of mRNA abundance despite protein synthesis inhibition and growth state

Jay E Purdy; John E Donelson; Mary E Wilson

doi:10.1016/j.exppara.2005.02.008

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Leishmania chagasi: The α-tubulin intercoding region results in constant levels of mRNA abundance despite protein synthesis inhibition and growth state

Journal article

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Leishmania chagasi: The α-tubulin intercoding region results in constant levels of mRNA abundance despite protein synthesis inhibition and growth state

Jay E Purdy, John E Donelson and Mary E Wilson

Experimental parasitology, Vol.110(2), pp.102-107

2005

DOI: 10.1016/j.exppara.2005.02.008

PMID: 15888291

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Abstract

The intercoding regions of many Leishmania sp. genes have been implicated in the regulation of mRNA processing, stability, and translation. Herein we show that the intercoding region of the Leishmania chagasi α- tubulin gene ( α- TUB) confers stable β- galactosidase ( β- GAL) reporter mRNA levels during promastigote growth and development in vitro and during protein synthesis inhibition. The abundance of both endogenous α- TUB mRNA and β- GAL mRNA from a β- GAL coding region situated upstream of the α- TUB intercoding region did not change significantly as promastigotes grew from logarithmic to stationary phase in vitro and the half-life of the β- GAL mRNA remained constant. The abundance of both the endogenous α- TUB and the β- GAL mRNA increased by less than 2-fold after protein synthesis inhibition corresponding to a moderate increase in mRNA half-life. These data suggest that the α- TUB intercoding region is an excellent control for the study of the regulation of other differentially expressed genes.

α- TUB

major surface protease logarithmic subclass

MSPL

T1/2

Gene regulation

α-tubulin

half-life

Cycloheximide

Leishmania chagasi

Intercoding region

β-galactosidase gene

β- GAL

mRNA stability

Details

Title: Subtitle: Leishmania chagasi: The α-tubulin intercoding region results in constant levels of mRNA abundance despite protein synthesis inhibition and growth state
Creators: Jay E Purdy - Department of Internal Medicine, University of Iowa, Iowa city, IA 52242, USA
John E Donelson - Department of Biochemistry, University of Iowa, Iowa city, IA 52242, USA
Mary E Wilson - Department of Internal Medicine, University of Iowa, Iowa city, IA 52242, USA
Resource Type: Journal article
Publication Details: Experimental parasitology, Vol.110(2), pp.102-107
Publisher: Elsevier Inc
DOI: 10.1016/j.exppara.2005.02.008
PMID: 15888291
ISSN: 0014-4894
eISSN: 1090-2449
Language: English
Date published: 2005
Academic Unit: Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984002398802771

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