Leishmania infantum chagasi: A genome-based approach to identification of excreted/secreted proteins

Sruti DebRoy; Alexandra B Keenan; Norikiyo Ueno; Selma M.B Jeronimo; John E Donelson; Mary E Wilson

doi:10.1016/j.exppara.2010.06.011

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Leishmania infantum chagasi: A genome-based approach to identification of excreted/secreted proteins

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Leishmania infantum chagasi: A genome-based approach to identification of excreted/secreted proteins

Sruti DebRoy, Alexandra B Keenan, Norikiyo Ueno, Selma M.B Jeronimo, John E Donelson and Mary E Wilson

Experimental parasitology, Vol.126(4), pp.582-591

2010

DOI: 10.1016/j.exppara.2010.06.011

PMCID: PMC2939245

PMID: 20542033

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https://www.ncbi.nlm.nih.gov/pmc/articles/2939245View

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Abstract

The parasitic protozoan, Leishmania, survives in harsh environments within its mammalian and sand fly hosts. Secreted proteins likely play critical roles in the parasite’s interactions with its environment. As a preliminary identification of the spectrum of potential excreted/secreted (ES) proteins of Leishmania infantum chagasi ( Lic), a causative agent of visceral leishmaniasis, we used standard algorithms to screen the annotated L. infantum genome for genes whose predicted protein products have an N-terminal signal peptide and lack transmembrane domains and membrane anchors. A suite of 181 candidate ES proteins were identified. These included several that were documented in the literature to be released by other Leishmania spp. Six candidate ES proteins were selected for further validation of their expression and release by different parasite stages. We found both amastigote-specific and promastigote-specific released proteins. The ES proteins of Lic are candidates for future studies of parasite virulence determinants and host protective immunity.

Excreted/secreted proteins

Trypanosomatid

Genome

Secretory pathway

Leishmania

Details

Title: Subtitle: Leishmania infantum chagasi: A genome-based approach to identification of excreted/secreted proteins
Creators: Sruti DebRoy - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
Alexandra B Keenan - Department of Biomedical Engineering, University of Iowa, Iowa City, IA 52242, USA
Norikiyo Ueno - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Selma M.B Jeronimo - Department of Biochemistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil
John E Donelson - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Mary E Wilson - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Experimental parasitology, Vol.126(4), pp.582-591
DOI: 10.1016/j.exppara.2010.06.011
PMID: 20542033
PMCID: PMC2939245
NLM abbreviation: Exp Parasitol
ISSN: 0014-4894
eISSN: 1090-2449
Publisher: Elsevier Inc
Language: English
Date published: 2010
Academic Unit: Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984001144302771

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