Journal article
Lentiviral Vector Gene Transfer to Porcine Airways
Molecular therapy. Nucleic acids, Vol.1(11), pp.e56-e56
11/2012
DOI: 10.1038/mtna.2012.47
PMCID: PMC3511674
PMID: 23187455
Abstract
In this study, we investigated lentiviral vector development and transduction efficiencies in well-differentiated primary cultures of pig airway epithelia (PAE) and wild-type pigs
in vivo
. We noted gene transfer efficiencies similar to that observed for human airway epithelia (HAE). Interestingly, feline immunodeficiency virus (FIV)-based vectors transduced immortalized pig cells as well as pig primary cells more efficiently than HIV-1–based vectors. PAE express TRIM5α, a well-characterized species-specific lentiviral restriction factor. We contrasted the restrictive properties of porcine TRIM5α against FIV- and HIV-based vectors using gain and loss of function approaches. We observed no effect on HIV-1 or FIV conferred transgene expression in response to porcine TRIM5α overexpression or knockdown. To evaluate the ability of GP64-FIV to transduce porcine airways
in vivo
, we delivered vector expressing mCherry to the tracheal lobe of the lung and the ethmoid sinus of 4-week-old pigs. One week later, epithelial cells expressing mCherry were readily detected. Our findings indicate that pseudotyped FIV vectors confer similar tropisms in porcine epithelia as observed in human HAE and provide further support for the selection of GP64 as an appropriate envelope pseudotype for future preclinical gene therapy studies in the porcine model of cystic fibrosis (CF).
Details
- Title: Subtitle
- Lentiviral Vector Gene Transfer to Porcine Airways
- Creators
- Patrick L Sinn - , Iowa City, IowaAshley L Cooney - , Iowa City, IowaMayumi Oakland - , Iowa City, IowaDouglas E Dylla - , Iowa City, IowaTanner J Wallen - , Iowa City, IowaAlejandro A Pezzulo - , Iowa City, IowaEugene H Chang - , Iowa City, IowaPaul B McCray - , Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Molecular therapy. Nucleic acids, Vol.1(11), pp.e56-e56
- DOI
- 10.1038/mtna.2012.47
- PMID
- 23187455
- PMCID
- PMC3511674
- NLM abbreviation
- Mol Ther Nucleic Acids
- ISSN
- 2162-2531
- eISSN
- 2162-2531
- Publisher
- Nature Publishing Group
- Language
- English
- Date published
- 11/2012
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Internal Medicine
- Record Identifier
- 9984093213102771
Metrics
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