Journal article
Lentiviral transduction of murine oligodendrocytes in vivo
Journal of neuroscience research, Vol.82(3), pp.397-403
11/01/2005
DOI: 10.1002/jnr.20626
PMID: 16158420
Abstract
Lentiviral vectors are used widely to direct efficient gene transfer in vivo. We examined cell-specific expression in adult murine white matter after stereotaxic microinjection of four lentiviral constructs. We synthesized vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviruses with combinations of two promoters, cytomegalovirus (CMV) or myelin basic protein (MBP), and two reporter sequences, cytosolic enhanced green fluorescent protein (eGFP) or a plasma membrane-targeted eGFP (human lymphocyte-specific protein tyrosine kinase [Lck]-eGFP). For all constructs, intracerebral injections to lateral corpus callosum resulted in wide-spread GFP expression in forebrain white matter glial cells. Intense cellular GFP fluorescence was observed within 3 days after injection and lasted for at least 28 days. The CMV promoter directed GFP expression in multiple glial cell types, whereas the MBP promoter targeted GFP specifically to oligodendrocytes. Expression of the membrane-targeted Lck-eGFP construct distinctly labeled individual myelinating processes of oligodendrocytes. Lentiviral constructs expressing eGFP or Lck-eGFP under the MBP promoter provide excellent visualization of oligodendrocyte morphology in intact white matter, and may prove valuable for delivering additional genes of interest to oligodendrocytes in vivo. © 2005 Wiley-Liss, Inc.
Details
- Title: Subtitle
- Lentiviral transduction of murine oligodendrocytes in vivo
- Creators
- Sally R McIver - Hope Center for Neurological Disorders, Washington University School of Medicine, St. Louis, MissouriChul-Sang Lee - Hope Center for Neurological Disorders, Washington University School of Medicine, St. Louis, MissouriJin-Moo Lee - Hope Center for Neurological Disorders, Washington University School of Medicine, St. Louis, MissouriSteven H Green - Department of Biological Sciences, University of Iowa, Iowa City, IowaMark S Sands - Hope Center for Neurological Disorders, Washington University School of Medicine, St. Louis, MissouriB. Joy Snider - Hope Center for Neurological Disorders, Washington University School of Medicine, St. Louis, MissouriMark P Goldberg - Hope Center for Neurological Disorders, Washington University School of Medicine, St. Louis, Missouri
- Resource Type
- Journal article
- Publication Details
- Journal of neuroscience research, Vol.82(3), pp.397-403
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/jnr.20626
- PMID
- 16158420
- ISSN
- 0360-4012
- eISSN
- 1097-4547
- Number of pages
- 7
- Language
- English
- Date published
- 11/01/2005
- Academic Unit
- Biology; Otolaryngology
- Record Identifier
- 9984217536202771
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