Journal article
Lewis Rat T Cells Can Reutilize, Process, and Present Myelin Basic Protein to Antigen-Specific T Cell Lines
Cellular immunology, Vol.156(1), pp.36-53
06/01/1994
DOI: 10.1006/cimm.1994.1151
PMID: 7515332
Abstract
MHC class-II-negative astrocytes prevented from intracellular antigen (Ag) processing induce myelin basic protein (MBP)-specific short-term T cell lines to proliferate. This process results from the ability of the T cells themselves to take up, process, and present Ag to each other. The Ag-presenting function of the T cells occurred in the absence of any conventional antigen-presenting cell (APC), was independent of their T cell receptor specificity, was sensitive to chloroquine, and was prevented by anti-class-II MHC antibody. Both native and HPLC-purified MBP were effective in stimulating T cell lines, and there was no obvious benefit in using either enzymatically digested or synthetic peptide preparations of the Ag. Furthermore, the Ag-presenting T cells could take up, reutilize, and re-present Ag adsorbed to the surface of histoincompatible astrocytes. Responding T cells activated by Ag-presenting T cells in the absence of other conventional APC were fully encephalitogenic upon transfer to syngeneic recipients. These results have relevance for understanding pathogenetic mechanisms in T cell-mediated autoimmunity in the central nervous system.
Details
- Title: Subtitle
- Lewis Rat T Cells Can Reutilize, Process, and Present Myelin Basic Protein to Antigen-Specific T Cell Lines
- Creators
- Joanne M. St. LouisJohn M. Pasick - Western UniversityColleen Stein - Western UniversityDavid Freeman - Western UniversityBhagirath Singh - Western UniversitySamuel Dales - Western UniversityGill H. Strejan - Western University
- Resource Type
- Journal article
- Publication Details
- Cellular immunology, Vol.156(1), pp.36-53
- Publisher
- Elsevier Inc
- DOI
- 10.1006/cimm.1994.1151
- PMID
- 7515332
- ISSN
- 0008-8749
- eISSN
- 1090-2163
- Language
- English
- Date published
- 06/01/1994
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984618641202771
Metrics
3 Record Views