Journal article
Light, Kindling, Action! Brain-wide Circuit Changes After Optogenetic Hippocampal Kindling Revealed by Functional Magnetic Resonance Imaging
Epilepsy currents, Vol.22(3), pp.190-191
03/26/2022
DOI: 10.1177/15357597221083405
PMCID: PMC9684594
PMID: 36474835
Abstract
Repeated seizure activity can lead to long-term changes in seizure dynamics and behavior. However, resulting changes in brainwide dynamics remain poorly understood. This is due partly to technical challenges in precise seizure control and in vivo wholebrain mapping of circuit dynamics. Here, we developed an optogenetic kindling model through repeated stimulation of ventral hippocampal CaMKII neurons in adult rats. We then combined fMRI with electrophysiology to track brain-wide circuit dynamics resulting from nonafterdischarge (AD)-generating stimulations and individual convulsive seizures. Kindling induced widespread increases in nonAD-generating stimulation response and ipsilateral functional connectivity and elevated anxiety. Individual seizures in kindled animals showed more significant increases in brain-wide activity and bilateral functional connectivity. Onset time quantification provided evidence for kindled seizure propagation from the ipsilateral to the contralateral hemisphere. Furthermore, a core of slow-migrating hippocampal activity was identified in both non-kindled and kindled seizures, revealing a novel mechanism of seizure sustainment and propagation.
Details
- Title: Subtitle
- Light, Kindling, Action! Brain-wide Circuit Changes After Optogenetic Hippocampal Kindling Revealed by Functional Magnetic Resonance Imaging
- Creators
- Gordon F Buchanan - University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Epilepsy currents, Vol.22(3), pp.190-191
- DOI
- 10.1177/15357597221083405
- PMID
- 36474835
- PMCID
- PMC9684594
- NLM abbreviation
- Epilepsy Curr
- ISSN
- 1535-7597
- eISSN
- 1535-7511
- Publisher
- Sage
- Number of pages
- 2
- Language
- English
- Date published
- 03/26/2022
- Academic Unit
- Neurology; Iowa Neuroscience Institute
- Record Identifier
- 9984242657002771
Metrics
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