Light spectra as triggers for sorting improved strains of Tisochrysis lutea

Fengzheng Gao; Sep Woolschot; Iago Teles Dominguez Cabanelas; Rene H. Wijffels; Maria J. Barbosa

doi:10.1016/j.biortech.2020.124434

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Light spectra as triggers for sorting improved strains of Tisochrysis lutea

Journal article

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Light spectra as triggers for sorting improved strains of Tisochrysis lutea

Fengzheng Gao, Sep Woolschot, Iago Teles Dominguez Cabanelas, Rene H. Wijffels and Maria J. Barbosa

Bioresource technology, Vol.321, pp.124434-124434

02/01/2021

DOI: 10.1016/j.biortech.2020.124434

PMID: 33257166

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https://doi.org/10.1016/j.biortech.2020.124434View

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Abstract

It is known that microalgae respond to different light colors, but not at single-cell level. This work aimed to assess if different light colors could be used as triggers to sort over-producing cells. Six light spectra were used: red + green + blue (RGBL), blue (BL), red (RL), green (GL), blue + red (BRL) and blue + green (BGL). Fluorescence-activated cell sorting method was used to analyse single-cell fluorescence and sort cells. BGL and RGBL lead to the highest fucoxanthin production, while RL showed the lowest. Therefore, it was hypothesized that hyperproducing cells can be isolated efficiently under the adverse condition (RL). After exposure to all light colors for 14 days, the top 1% fucoxanthin producing cells were sorted. A sorted strain from RL showed higher (16-19%) growth rate and fucoxanthin productivity. This study showed how light spectra affected single-cell fucoxanthin and lipid contents and productivities. Also, it supplied an approach to sort for high-fucoxanthin or high-lipid cells.

Agricultural Engineering

Agriculture

Biotechnology & Applied Microbiology

Energy & Fuels

Life Sciences & Biomedicine

Science & Technology

Technology

Details

Title: Subtitle: Light spectra as triggers for sorting improved strains of Tisochrysis lutea
Creators: Fengzheng Gao - Wageningen University & Research
Sep Woolschot - Wageningen University & Research
Iago Teles Dominguez Cabanelas - Wageningen University & Research
Rene H. Wijffels - Nord University
Maria J. Barbosa - Wageningen Univ, Bioproc Engn, AlgaePARC, POB 16, NL-6700 AA Wageningen, Netherlands
Resource Type: Journal article
Publication Details: Bioresource technology, Vol.321, pp.124434-124434
DOI: 10.1016/j.biortech.2020.124434
PMID: 33257166
NLM abbreviation: Bioresour Technol
ISSN: 0960-8524
eISSN: 1873-2976
Publisher: Elsevier
Number of pages: 10
Grant note: 745754 / Bio Based Industries Joint Undertaking under the European Union's Horizon 2020 research and innovation program
Language: English
Date published: 02/01/2021
Academic Unit: International Programs; Spanish and Portuguese
Record Identifier: 9984398019402771

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