Journal article
Lin− Cells Mediate Tissue Repair by Regulating MCP-1/CCL-2
The American journal of pathology, Vol.177(4), pp.2002-2010
10/2010
DOI: 10.2353/ajpath.2010.091232
PMCID: PMC2947294
PMID: 20813969
Abstract
Exogenous bone marrow-derived cells (BMDCs) are promising therapeutic agents for the treatment of tissue ischemia and traumatic injury. However, until we identify the molecular mechanisms that underlie their actions, there can be no rational basis for the design of therapeutic strategies using BMDCs. The pro-healing effects of BMDCs are apparent very shortly after treatment, which suggests that they may exert their effects by the modulation of acute inflammation. We investigated this hypothesis by taking advantage of the fact that BMDCs from healthy, young, but not obese, diabetic mice stimulate vascular growth. By comparing both
in vitro
secretion and
in vivo
local induction of acute phase inflammatory cytokines by these cells, we identified monocyte chemoattractant factor 1 and tumor necrosis factor α as potential mediators of BMDC-induced tissue repair.
In vivo
analysis of BMDC-treated ischemic limbs and cutaneous wounds revealed that the production of monocyte chemoattractant factor 1 by exogenous and endogenous BMDCs is essential for BMDC-mediated vascular growth and tissue healing, while the inability of BMDCs to produce tumor necrosis factor α appears to play a lesser but still meaningful role. Thus, measurements of the secretion of cytokines by BMDCs may allow us to identify
a priori
individuals who would or would not be good candidates for BMDC-based therapies.
Details
- Title: Subtitle
- Lin− Cells Mediate Tissue Repair by Regulating MCP-1/CCL-2
- Creators
- Gina C Schatteman - Department of Integrative Physiology, The University of Iowa, Iowa City, IowaOla Awad - Department of Integrative Physiology, The University of Iowa, Iowa City, IowaEric Nau - Department of Integrative Physiology, The University of Iowa, Iowa City, IowaChunlin Wang - Department of Integrative Physiology, The University of Iowa, Iowa City, IowaChunhua Jiao - Department of Integrative Physiology, The University of Iowa, Iowa City, IowaRobert J Tomanek - Departments of Anatomy and Cell Biology, The University of Iowa, Iowa City, IowaMartine Dunnwald - Department of Pediatrics, The University of Iowa, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- The American journal of pathology, Vol.177(4), pp.2002-2010
- DOI
- 10.2353/ajpath.2010.091232
- PMID
- 20813969
- PMCID
- PMC2947294
- NLM abbreviation
- Am J Pathol
- ISSN
- 0002-9440
- eISSN
- 1525-2191
- Publisher
- American Society for Investigative Pathology
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: AR055313, DK55965, DK59223
- Language
- English
- Date published
- 10/2010
- Academic Unit
- Anatomy and Cell Biology; Craniofacial Anomalies Research Center; Health, Sport, and Human Physiology ; Ophthalmology and Visual Sciences
- Record Identifier
- 9984025476702771
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