Lineage-specific regulation of the Ciona snail gene in the embryonic mesoderm and neuroectoderm

A Erives; J C Corbo; M Levine

doi:10.1006/dbio.1997.8810

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Lineage-specific regulation of the Ciona snail gene in the embryonic mesoderm and neuroectoderm

Journal article

Open access

Peer reviewed

Lineage-specific regulation of the Ciona snail gene in the embryonic mesoderm and neuroectoderm

A Erives, J C Corbo and M Levine

Developmental biology, Vol.194(2), pp.213-225

02/15/1998

DOI: 10.1006/dbio.1997.8810

PMID: 9501022

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Abstract

The snail gene encodes a highly conserved, zinc-finger transcription factor that has been implicated in the specification of mesodermal and neuronal tissues in a variety of organisms. In the ascidian, Ciona intestinalis, snail (Ci-sna) is expressed at the 32-cell stage in derivatives of the B4.1 blastomere, including B6.2, B6.4, and B7.5, which give rise to the primary-lineage tail muscles of the tadpole. At later stages, Ci-sna is expressed in the lineages that will form the secondary tail muscle, the lateral ependymal cells of the spinal cord, and the dorsal cells of the cerebral vesicle. A minimal, 504-bp cis-regulatory sequence from the Ci-sna promoter region, the B4.1 enhancer, is shown to direct the expression of heterologous promoters in primary-lineage muscles. Furthermore, evidence is presented that cis-regulatory elements necessary for expression in both the secondary muscle and neuronal lineages are separate from the B4.1 enhancer. We discuss the possibility that the classical muscle determinant present in ascidian eggs may correspond to bHLH activators, which bind to specific E-box sequences contained in the B4.1 enhancer.

Animals

Base Sequence

Ciona intestinalis - embryology

DNA-Binding Proteins - genetics

Ectoderm - physiology

Gene Expression Regulation, Developmental

In Situ Hybridization

Lac Operon

Mesoderm - physiology

Molecular Sequence Data

Muscles - embryology

Snail Family Transcription Factors

Transcription Factors

Details

Title: Subtitle: Lineage-specific regulation of the Ciona snail gene in the embryonic mesoderm and neuroectoderm
Creators: A Erives - Department of Molecular and Cellular Biology, University of California, Berkeley 94720, USA. aerives@alumni.caltech.edu
J C Corbo
M Levine
Resource Type: Journal article
Publication Details: Developmental biology, Vol.194(2), pp.213-225
DOI: 10.1006/dbio.1997.8810
PMID: 9501022
NLM abbreviation: Dev Biol
ISSN: 0012-1606
eISSN: 1095-564X
Language: English
Date published: 02/15/1998
Academic Unit: Biology
Record Identifier: 9984217426602771

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