Journal article
Linoleic acid amplifies polychlorinated biphenyl-mediated dysfunction of endothelial cells
Journal of biochemical and molecular toxicology, Vol.13(2), pp.83-91
1999
DOI: 10.1002/(SICI)1099-0461(1999)13:2<83::AID-JBT4>3.0.CO;2-7
PMID: 9890193
Abstract
Selected dietary lipids may increase the atherogenicity of environmental chemicals, such as polychlorinated biphenyls (PCBs), by cross-amplifying mechanisms leading to dysfunction of the vascular endothelium. To investigate this hypothesis, cultured endothelial cells were treated with 90 microM linoleic acid (18:2n-6), followed by either one of two PCBs, 3,3',4,4'-tetrachlorobiphenyl (PCB 77) or 2,2'4,4',5,5'-hexachlorobiphenyl (PCB 153). These PCBs were selected for their varying binding activities with the aryl hydrocarbon (Ah) receptor and differences in their induction of cytochrome P450. PCB 77 disrupted endothelial barrier function by allowing an increase in albumin transfer across endothelial monolayers. Prior cellular enrichment with 18:2 before PCB treatment further diminished endothelial barrier function, as compared to cells treated only with the PCB. This phenomenon appears to be mediated by increased oxidative stress, which is supported by enhanced 2,7-dichlorofluorescein fluorescence, activation data of the oxidative stress-sensitive nuclear transcription factor-kappaB (NF-kappaB), as well as an observed decrease in vitamin E content in the culture media. Similar to the endothelial permeability data, pre-enrichment of cells with 18:2 further increased the PCB-mediated induction of cytochrome P450 1A. In contrast to PCB 77, PCB 153 (or 18:2 plus PCB 153) had little or no effect on endothelial barrier function. Our results suggest that certain unsaturated fatty acids can potentiate PCB-mediated endothelial cell dysfunction and that oxidative stress and activation of the cytochrome P450 1A subfamily may be, in part, responsible for these metabolic events. These findings have implications for understanding the involvement of certain environmental contaminants in diseases that involve dysfunction of the vascular endothelium.
Details
- Title: Subtitle
- Linoleic acid amplifies polychlorinated biphenyl-mediated dysfunction of endothelial cells
- Creators
- Bernhard Hennig - Department of Nutrition and Food Science, Graduate Center for Toxicology, University of Kentucky, Lexington 40506-0054, USAR SlimM ToborekL W Robertson
- Resource Type
- Journal article
- Publication Details
- Journal of biochemical and molecular toxicology, Vol.13(2), pp.83-91
- Publisher
- United States
- DOI
- 10.1002/(SICI)1099-0461(1999)13:2<83::AID-JBT4>3.0.CO;2-7
- PMID
- 9890193
- ISSN
- 1095-6670
- eISSN
- 1099-0461
- Grant note
- P42 ES007380 / NIEHS NIH HHS 1 P42 ES 07380 / NIEHS NIH HHS
- Language
- English
- Date published
- 1999
- Academic Unit
- Occupational and Environmental Health
- Record Identifier
- 9984002454002771
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