Journal article
Lipid bodies accumulation in Leishmania infantum-infected C57BL/6 macrophages
Parasite immunology, Vol.39(8), pp.e12443-n/a
08/2017
DOI: 10.1111/pim.12443
PMCID: PMC5512551
PMID: 28518475
Abstract
Lipid bodies (LBs) are intracellular accumulations of neutral lipids surrounded by a single membrane. These organelles are involved in the production of eicosanoids, which modulate immunity by either promoting or dampening inflammatory responses.
Leishmania infantum
, the etiological agent of visceral leishmaniasis in Brazil, is an intracellular parasite that causes disease by suppressing macrophage microbicidal responses. C57BL/6 mouse bone marrow-derived macrophages infected with
L. infantum
strain LcJ had higher numbers of LB+ cells (P<0.0001) and total LBs than non-infected cultures. Large (> 3μm) LBs were present inside parasitophorous vacuoles (PVs). These results contrast with those of
L. infantum
-infected BALB/c macrophages, in which the only LBs are derived from parasite, not macrophage origin. Increased LBs in C57BL/6 macrophages in close association with parasites would position host LBs where they could modulate
L. infantum
infection. These results imply a potential influence of the host genetics on the role of LBs in host-pathogen interactions. Overall, our data support a model in which the expression, and the role of LBs upon infection, ultimately depends of the specific combination of host-pathogen interactions.
Details
- Title: Subtitle
- Lipid bodies accumulation in Leishmania infantum-infected C57BL/6 macrophages
- Creators
- Nilda E Rodríguez - Veterans’ Affairs Medical Center, Iowa City, IA, USARyan D Lockard - Veterans’ Affairs Medical Center, Iowa City, IA, USAElizabeth A Turcotte - Veterans’ Affairs Medical Center, Iowa City, IA, USAThéo Araujo-Santos - Veterans’ Affairs Medical Center, Iowa City, IA, USAPatrícia T Bozza - Veterans’ Affairs Medical Center, Iowa City, IA, USAValéria M Borges - Veterans’ Affairs Medical Center, Iowa City, IA, USAMary E Wilson - Veterans’ Affairs Medical Center, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Parasite immunology, Vol.39(8), pp.e12443-n/a
- DOI
- 10.1111/pim.12443
- PMID
- 28518475
- PMCID
- PMC5512551
- ISSN
- 0141-9838
- eISSN
- 1365-3024
- Grant note
- DOI: 10.13039/100010179, name: University of Northern Iowa; DOI: 10.13039/100000002, name: National Institutes of Health, award: AI076233, AI045540; DOI: 10.13039/100000738, name: U.S. Department of Veterans Affairs, award: 5I01BX001983, 2I01BX000536
- Language
- English
- Date published
- 08/2017
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Internal Medicine
- Record Identifier
- 9984001205002771
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