Journal article
Lipid nanoparticle-mRNA: another step in the fight against COVID-19
Cell research, Vol.32(4), pp.421-422
03/21/2022
DOI: 10.1038/s41422-022-00647-5
PMCID: PMC8936036
PMID: 35314770
Abstract
Monoclonal antibodies represent important weapons in our arsenal to against the COVID-19 pandemic. However, this potential is severely limited by the time-consuming process of developing effective antibodies and the relative high cost of manufacturing. Herein, we present a rapid and cost-effective lipid nanoparticle (LNP) encapsulated-mRNA platform for in vivo delivery of SARS-CoV-2 neutralization antibodies. Two mRNAs encoding the light and heavy chains of a potent SARS-CoV-2 neutralizing antibody HB27, which is currently being evaluated in clinical trials, were encapsulated into clinical grade LNP formulations (named as mRNA-HB27-LNP). In vivo characterization demonstrated that intravenous administration of mRNA-HB27-LNP in mice resulted in a longer circulating half-life compared with the original HB27 antibody in protein format. More importantly, a single prophylactic administration of mRNA-HB27-LNP provided protection against SARS-CoV-2 challenge in mice at 1, 7 and even 63 days post administration. In a close contact transmission model, prophylactic administration of mRNA-HB27-LNP prevented SARS-CoV-2 infection between hamsters in a dose-dependent manner. Overall, our results demonstrate a superior long-term protection against SARS-CoV-2 conferred by a single administration of this unique mRNA antibody, highlighting the potential of this universal platform for antibody-based disease prevention and therapy against COVID-19 as well as a variety of other infectious diseases.
Details
- Title: Subtitle
- Lipid nanoparticle-mRNA: another step in the fight against COVID-19
- Creators
- Abhishek Kumar Verma - University of IowaStanley Perlman - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Cell research, Vol.32(4), pp.421-422
- DOI
- 10.1038/s41422-022-00647-5
- PMID
- 35314770
- PMCID
- PMC8936036
- eISSN
- 1748-7838
- Language
- English
- Date published
- 03/21/2022
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
- Record Identifier
- 9984229996602771
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