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Lipooligosaccharide Ligands from Respiratory Bacterial Pathogens Enhance Cellular Uptake of Nanoparticles
Journal article   Open access   Peer reviewed

Lipooligosaccharide Ligands from Respiratory Bacterial Pathogens Enhance Cellular Uptake of Nanoparticles

Mai H. Tu, Timothy M. Brenza, Margaret R. Ketterer, Morgan Timm, Benjamin M. King, Michael A. Apicella and Jennifer Fiegel
KONA Powder and Particle Journal, Vol.41, pp.265-279
01/10/2024
DOI: 10.14356/kona.2024005
url
https://doi.org/10.14356/kona.2024005View
Published (Version of record) Open Access

Abstract

Several bacterial pathogens contain membrane ligands that facilitate their binding and internalization into human tissues. In this study, lipooligosaccharides (LOS) from the respiratory pathogen non-typeable Haemophilus influenzae (NTHi) were isolated from the bacterial surface and evaluated as a nanoparticle coating material to facilitate uptake into respiratory epithelium. NTHi clinical isolates were screened to select a strain with high binding potential due to their elevated phosphorylcholine content. The association of particles with human bronchial epithelial cells was investigated as a function of particle surface chemistry and incubation time, and the uptake mechanism evaluated via chemical inhibitor and receptor activation studies. A more than two-fold enhancement in particle uptake was achieved by coating the particles with LOS compared to uncoated or gelatin-coated particles, which was further increased by activating the platelet activating factor receptor (PAFR). These findings demonstrate that bacterial-derived LOS ligands can enhance the targeting and binding of nanoparticles to lung epithelial cells.
 bronchial epithelial cells endocytosis lipooligosaccharides nanoparticle non-typeable Haemophilus influenzae platelet-activating factor receptor

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