Journal article
Lipopolysaccharide Temporarily Impairs Sphincter of Oddi Motility
Nitric oxide, Vol.5(6), pp.547-554
12/2001
DOI: 10.1006/niox.2001.0380
PMID: 11730361
Abstract
The aim of our study was to determine the effect of lipopolysaccharide (LPS) on sphincter of Oddi (SO) motility. Opossums received saline, Escherichia coli LPS (1.0 mg/kg), or E. coli LPS (1.0 mg/kg) and aminoguanidine (50 mg/kg), and the SO was removed 6–24 h later. At 12 h LPS decreased electrical field stimulation (EFS)-induced relaxation and increased baseline tone. These changes were reversed when the animals were pretreated with aminoguanidine. The dose-dependent decrease in EFS-induced relaxation by Nω-nitro-l-arginine was impaired after LPS, but not in animals that received LPS and aminoguanidine. The impaired EFS-induced relaxation after LPS was reversed when l-arginine was added to the tissue bath. Serum levels of NO−2/NO−3 were increased with LPS as compared to saline or both LPS and aminoguanidine. Inducible nitric oxide synthase mRNA was readily seen in SO segments after LPS. LPS impairs EFS-induced relaxation and increases baseline tone of the SO. The effects of LPS on SO motility appear to be mediated by nitric oxide.
Details
- Title: Subtitle
- Lipopolysaccharide Temporarily Impairs Sphincter of Oddi Motility
- Creators
- Joseph J Cullen - Departments of Surgery, University of Iowa College of Medicine and Veterans Affairs Medical Center, Iowa City, Iowa, 52242Hyojin Park - Departments of Internal Medicine, University of Iowa College of Medicine and Veterans Affairs Medical Center, Iowa City, Iowa, 52242Kimberly S Ephgrave - Departments of Surgery, University of Iowa College of Medicine and Veterans Affairs Medical Center, Iowa City, Iowa, 52242Jeffrey L Conklin - Departments of Internal Medicine, University of Iowa College of Medicine and Veterans Affairs Medical Center, Iowa City, Iowa, 52242
- Resource Type
- Journal article
- Publication Details
- Nitric oxide, Vol.5(6), pp.547-554
- DOI
- 10.1006/niox.2001.0380
- PMID
- 11730361
- NLM abbreviation
- Nitric Oxide
- ISSN
- 1089-8603
- eISSN
- 1089-8611
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 12/2001
- Academic Unit
- Surgery; Radiation Oncology
- Record Identifier
- 9984051709402771
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