Journal article
Lipopolysaccharide-induced preconditioning protects against traumatic spinal cord injury by upregulating Nrf2 expression in rats
Life sciences (1973), Vol.162, pp.14-20
10/01/2016
DOI: 10.1016/j.lfs.2016.08.008
PMID: 27515502
Abstract
Aims: Lipopolysaccharide (LPS)-induced preconditioning protects neurons against traumatic spinal cord injury (TSCI) in rats. This study sought to test whether Nrf2, a transcription factor, mediated LPS-induced preconditioning.
Main methods: The TSCI model was established using a standardized NYU impactor on adult female rats. Rats were pretreated with LPS (0.2 mg/kg, IP; 72 h before injury). Nrf2 was silenced by injecting a lentivirus encoding RNAi against Nrf2 into injured spinal cords. Neurologic function was assessed by Basso, Beattie and Bresnahan (BBB) scores 6 h, 12 h, 24 h, 48 h, 72 h, 7 d and 14 d after TSCI. Neuronal apoptosis was measured by a TUNEL staining. Ultrastructure was observed using transmission electron microscope (TEM). The protein expression of HO-1, NQO1 and GCLC was examined using immunohistochemistry and immunoblotting.
Key findings: The injection of a lentivirus effectively transfected GFP into injured spinal cords. The expression of Nrf2 was significantly decreased in spinal cords receiving a lentivirus encoding RNAi against Nrf2. BBB scores showed that TSCI markedly impaired nervous function, which was markedly preserved by LPS pretreatment. Nrf2 knockdown significantly suppressed LPS pretreatment-induced protection of nervous function. TEM images and TUNEL staining showed an increase in apoptotic cells when Nrf2 was silenced. Moreover, immunohistochemistry and immunoblotting analysis exhibited that LPS pretreatment significantly upregulated the expression of anti-oxidative proteins including HO-1, NQ01 and GCLC, which was suppressed when Nrf2 expression was silenced in injured spinal cords.
Significance: LPS preconditioning effectively alleviates TSCI-induced impairment and preserves nervous function in a Nrf2-dependent manner. (C) 2016 Elsevier Inc. All rights reserved.
Details
- Title: Subtitle
- Lipopolysaccharide-induced preconditioning protects against traumatic spinal cord injury by upregulating Nrf2 expression in rats
- Creators
- Weichao Li - Kunming University of Science and TechnologyDianming Jiang - First Affiliated Hospital of Chongqing Medical UniversityQiuxia Li - University of IowaShaoping Yao - Kunming University of Science and TechnologyXianrun Sun - Kunming University of Science and TechnologyYong Yang - Kunming University of Science and TechnologyZengdong Meng - Kunming University of Science and TechnologyWei Liu - Kunming University of Science and Technology
- Resource Type
- Journal article
- Publication Details
- Life sciences (1973), Vol.162, pp.14-20
- Publisher
- Elsevier
- DOI
- 10.1016/j.lfs.2016.08.008
- PMID
- 27515502
- ISSN
- 0024-3205
- eISSN
- 1879-0631
- Number of pages
- 7
- Grant note
- 2014BS008 / Science Foundation of First People's Hospital of Yunnan Province KKSY201560051 / Development Program of Kunming University of Science and Technology
- Language
- English
- Date published
- 10/01/2016
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984359946302771
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