Liquid Biopsy Proteomics in Ophthalmology

Julian Wolf; Joel A Franco; Rui Yip; Mohamed Ziad Dabaja; Gabriel Velez; Fel Liu; Alexander G Bassuk; Prithvi Mruthyunjaya; Antoine Dufour; Vinit B Mahajan

doi:10.1021/acs.jproteome.3c00756

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Liquid Biopsy Proteomics in Ophthalmology

Journal article

Open access

Peer reviewed

Liquid Biopsy Proteomics in Ophthalmology

Julian Wolf, Joel A Franco, Rui Yip, Mohamed Ziad Dabaja, Gabriel Velez, Fel Liu, Alexander G Bassuk, Prithvi Mruthyunjaya, Antoine Dufour and Vinit B Mahajan

Journal of proteome research, Vol.23(2), pp.511-522

02/02/2024

DOI: 10.1021/acs.jproteome.3c00756

PMCID: PMC10845144

PMID: 38171013

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Abstract

Minimally invasive liquid biopsies from the eye capture locally enriched fluids that contain thousands of proteins from highly specialized ocular cell types, presenting a promising alternative to solid tissue biopsies. The advantages of liquid biopsies include sampling the eye without causing irreversible functional damage, potentially better reflecting tissue heterogeneity, collecting samples in an outpatient setting, monitoring therapeutic response with sequential sampling, and even allowing examination of disease mechanisms at the cell level in living humans, an approach that we refer to as TEMPO (Tracing Expression of Multiple Protein Origins). Liquid biopsy proteomics has the potential to transform molecular diagnostics and prognostics and to assess disease mechanisms and personalized therapeutic strategies in individual patients. This review addresses opportunities, challenges, and future directions of high-resolution liquid biopsy proteomics in ophthalmology, with particular emphasis on the large-scale collection of high-quality samples, cutting edge proteomics technology, and artificial intelligence-supported data analysis.

eye

liquid biopsy proteomics

vitreous

precision medicine

aqueous humor

artificial intelligence

Details

Title: Subtitle: Liquid Biopsy Proteomics in Ophthalmology
Creators: Julian Wolf
Joel A Franco - Stanford University
Rui Yip - Stanford University
Mohamed Ziad Dabaja - University of Calgary
Gabriel Velez - Stanford University
Fel Liu - Brigham and Women's Hospital
Alexander G Bassuk - University of Iowa
Prithvi Mruthyunjaya - Stanford University
Antoine Dufour
Vinit B Mahajan
Resource Type: Journal article
Publication Details: Journal of proteome research, Vol.23(2), pp.511-522
DOI: 10.1021/acs.jproteome.3c00756
PMID: 38171013
PMCID: PMC10845144
NLM abbreviation: J Proteome Res
eISSN: 1535-3907
Grant note: DOI: 10.13039/100000053, name: National Eye Institute, award: R01EY031360, R01EY031952, R01EY030151, P30EY026877; DOI: 10.13039/100001818, name: Research to Prevent Blindness; DOI: 10.13039/100018133, name: VitreoRetinal Surgery Foundation; DOI: 10.13039/100020710, name: Translational Research and Applied Medicine, School of Medicine, Stanford University; name: The Alan and Irene Adler Ocular Cancer Research Initiative; name: Stanford Center for Optic Disc Drusen
Language: English
Electronic publication date: 01/03/2024
Date published: 02/02/2024
Academic Unit: Neurology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Neurology (Pediatrics)
Record Identifier: 9984539750202771

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