Journal article
Liquid-Exfoliated Mesostructured Collagen from the Bovine Achilles Tendon as Building Blocks of Collagen Membranes
ACS applied materials & interfaces, Vol.13(2), pp.3186-3198
01/20/2021
DOI: 10.1021/acsami.0c20330
PMID: 33398989
Abstract
Mesoscaled assemblies are organized in native collagen tissues to achieve remarkable and diverse performance and functions. In this work, a facile, low-cost, and controllable liquid exfoliation method was applied to directly extract these collagen mesostructures from bovine Achilles tendons using a sodium hydroxide (NaOH)/urea aqueous system with freeze-thaw cycles and sonication. A series of collagen fibrils with diameters of 26-230 nm were harvested using this process, and in situ observations under polarizing microscopy (POM) and using molecular dynamics simulations revealed the influence of the NaOH/urea system on the tendon collagen. FTIR and XRD results confirmed that these collagen fibrils preserved typical structural characteristics of type I collagen. These isolated collagen fibrils were then utilized as building blocks to fabricate free-standing collagen membranes, which exhibited good stability in solvents and outstanding mechanical properties and transparency, with potential for utility in optical and electronic sensors. Moreover, in vitro and vivo evaluations demonstrated that these new resulting collagen membranes had good cytocompatibility, biocompatibility, and degradability for potential applications in biomedicine. This work provides a new approach for collagen processing by liquid exfoliation with utility for the formation of robust collagen materials that consist of native collagen mesostructures as building blocks.
Details
- Title: Subtitle
- Liquid-Exfoliated Mesostructured Collagen from the Bovine Achilles Tendon as Building Blocks of Collagen Membranes
- Creators
- Ying Pei - Zhengzhou UniversityKathryn E Jordan - Tufts UniversityNing Xiang - Tufts UniversityRachael N Parker - Tufts UniversityXuan Mu - Tufts UniversityLuan Zhang - Zhengzhou UniversityZhibin Feng - Zhengzhou UniversityYing Chen - Tufts UniversityChunmei Li - Tufts UniversityChengchen Guo - Tufts UniversityKeyong Tang - Zhengzhou UniversityDavid L Kaplan - Tufts University
- Resource Type
- Journal article
- Publication Details
- ACS applied materials & interfaces, Vol.13(2), pp.3186-3198
- DOI
- 10.1021/acsami.0c20330
- PMID
- 33398989
- ISSN
- 1944-8244
- eISSN
- 1944-8252
- Grant note
- DOI: 10.13039/100000070, name: National Institute of Biomedical Imaging and Bioengineering, award: P41EB027062; DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: 51603191
- Language
- English
- Date published
- 01/20/2021
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984277265502771
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