Journal article
Listeria monocytogenes infection overcomes the requirement for CD40 ligand in exogenous antigen presentation to CD8(+) T cells
The Journal of immunology (1950), Vol.167(10), pp.5603-5609
11/15/2001
DOI: 10.4049/jimmunol.167.10.5603
PMID: 11698431
Abstract
In vivo priming of CD8(+) T lymphocytes against exogenously processed model Ags requires CD4(+) T cell help, specifically interactions between CD40 ligand (CD40L) expressed by activated CD4(+) T cells and CD40, which is present on professional APC such as dendritic cells (DCs). To address this issue in the context of bacterial infection, we examined CD40L-CD40 interactions in CD8(+) T cell priming against an exogenously processed, nonsecreted bacterial Ag. CD40L interactions were blocked by in vivo treatment with anti-CD40L mAb MR-1, which inhibited germinal center formation and CD8(+) T cell cross-priming against an exogenous model Ag, OVA. In contrast, MR-1 treatment did not interfere with CD8(+) T cell priming against a nonsecreted or secreted recombinant Ag expressed by Listeria monocytogenes. Memory and secondary responses of CD8(+) T cells against nonsecreted and secreted bacterial Ags were also largely unimpaired by transient MR-1 treatment. When MR-1-treated mice were concurrently immunized with L. monocytogenes and OVA-loaded splenocytes, cross-priming of OVA-specific naive CD8(+) T cells occurred. No significant decline in cross-priming against OVA was measured when either TNF or IFN-gamma was neutralized in L. monocytogenes-infected animals, demonstrating that multiple signals exist to overcome CD40L blockade of CD8(+) T cell cross-priming during bacterial infection. These data support a model in which DCs can be stimulated in vivo through signals other than CD40, becoming APC that can effectively stimulate CD8(+) T cell responses against exogenous Ags during infection.
Details
- Title: Subtitle
- Listeria monocytogenes infection overcomes the requirement for CD40 ligand in exogenous antigen presentation to CD8(+) T cells
- Creators
- Sara E Hamilton - Interdisciplinary Program in Immunology, University of Iowa, Iowa City, IA 52242, USAAmy R TvinnereimJohn T Harty
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.167(10), pp.5603-5609
- Publisher
- United States
- DOI
- 10.4049/jimmunol.167.10.5603
- PMID
- 11698431
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Grant note
- AI42767 / NIAID NIH HHS AI50073 / NIAID NIH HHS 5T32A107485 / PHS HHS AI46653 / NIAID NIH HHS
- Language
- English
- Date published
- 11/15/2001
- Academic Unit
- Pathology
- Record Identifier
- 9984047758002771
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