Journal article
Lmo1656 is a secreted virulence factor of Listeria monocytogenes that interacts with the sorting nexin 6-BAR complex
The Journal of biological chemistry, Vol.293(24), pp.9265-9276
06/15/2018
DOI: 10.1074/jbc.RA117.000365
PMID: 29666193
Abstract
(
) is a facultative intracellular bacterial pathogen and the causative agent of listeriosis, a rare but fatal disease. During infection,
can traverse several physiological barriers; it can cross the intestine and placenta barrier and, in immunocompromised individuals, the blood-brain barrier. With the recent plethora of sequenced genomes available for
, it is clear that the complete repertoire of genes used by
to interact with its host remains to be fully explored. Recently, we focused on secreted
proteins because they are likely to interact with host cell components. Here, we investigated a putatively secreted protein of
, Lmo1656, that is present in most sequenced strains of
but absent in the nonpathogenic species
gene is predicted to encode a small, positively charged protein. We show that Lmo1656 is secreted by
Furthermore, deletion of the
gene (Δ
) attenuates virulence in mice infected orally but not intravenously, suggesting that Lmo1656 plays a role during oral listeriosis. We identified sorting nexin 6 (SNX6), an endosomal sorting component and BAR domain-containing protein, as a host cell interactor of Lmol656. SNX6 colocalizes with WT
during the early steps of infection. This colocalization depends on Lmo1656, and RNAi of SNX6 impairs infection in infected tissue culture cells, suggesting that SNX6 is utilized by
during infection. Our results reveal that Lmo1656 is a novel secreted virulence factor of
that facilitates recruitment of a specific member of the sorting nexin family in the mammalian host.
Details
- Title: Subtitle
- Lmo1656 is a secreted virulence factor of Listeria monocytogenes that interacts with the sorting nexin 6-BAR complex
- Creators
- Daryl Jason David - From the Unité des Interactions Bactéries-Cellules, Department of Cell Biology and Infection, INSERM U604, Institut National de la Recherche Agronomique USC2020, Institut Pasteur, 25 rue du Dr. Roux, 75015 Paris, FranceAlessandro Pagliuso - From the Unité des Interactions Bactéries-Cellules, Department of Cell Biology and Infection, INSERM U604, Institut National de la Recherche Agronomique USC2020, Institut Pasteur, 25 rue du Dr. Roux, 75015 Paris, FranceLilliana Radoshevich - From the Unité des Interactions Bactéries-Cellules, Department of Cell Biology and Infection, INSERM U604, Institut National de la Recherche Agronomique USC2020, Institut Pasteur, 25 rue du Dr. Roux, 75015 Paris, FranceMarie-Anne Nahori - From the Unité des Interactions Bactéries-Cellules, Department of Cell Biology and Infection, INSERM U604, Institut National de la Recherche Agronomique USC2020, Institut Pasteur, 25 rue du Dr. Roux, 75015 Paris, FrancePascale Cossart - From the Unité des Interactions Bactéries-Cellules, Department of Cell Biology and Infection, INSERM U604, Institut National de la Recherche Agronomique USC2020, Institut Pasteur, 25 rue du Dr. Roux, 75015 Paris, France pascale.cossart@pasteur.fr
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.293(24), pp.9265-9276
- DOI
- 10.1074/jbc.RA117.000365
- PMID
- 29666193
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- United States
- Language
- English
- Date published
- 06/15/2018
- Academic Unit
- Molecular Physiology and Biophysics; Microbiology and Immunology
- Record Identifier
- 9984025376002771
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