Journal article
Localization and characterization of a novel secreted protein SCUBE1 in human platelets
Cardiovascular research, Vol.71(3), pp.486-495
2006
DOI: 10.1016/j.cardiores.2006.04.010
PMID: 16753137
Abstract
Objective: The aim of the study was to investigate the protein expression and function of a novel secreted protein in the vascular system, named SCUBE1 for signal peptide, CUB (Complement proteins C1r/C1s, Uegf, and Bmp1) and epidermal growth factor-like (EGF)-like domain containing protein 1.
Methods and results Immunohistochemical analysis demonstrated that the SCUBE1 staining is mainly confined to the intravascular platelet-rich thrombus in vascular tissue samples. While quantitative real-time RT-PCR verified that the SCUBE1 mRNA is expressed in human platelets, numerous immunolocalization techniques revealed that the preformed SCUBE1 protein is stored in the α-granules and translocated to the surface upon platelet stimulation. A smaller SCUBE1 fragment, possibly formed by limited proteolysis after being released from the storage granules, was detected in thrombus lysate by Western blot analysis. Interestingly, deposition of SCUBE1 into the subendothelial matrix of the atherosclerotic plaques was evidenced by immunohistochemistry. In addition, studies of platelet adhesion and ristocetin-induced platelet agglutination showed that fragments containing the amino-terminal EGF-like repeats were able to support platelet adhesion and enhance the ristocetin-induced platelet agglutination, respectively.
Conclusion These data suggest that platelet-derived SCUBE1 could function as a novel adhesive molecule and its matrix-bound and soluble fragments may play critical (patho)physiological roles in cardiovascular biology.
Details
- Title: Subtitle
- Localization and characterization of a novel secreted protein SCUBE1 in human platelets
- Creators
- Cheng-Fen TU - Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Province of ChinaYueh-Hsing SU - Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Province of ChinaYa-Ni HUANG - Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Province of ChinaMing-Tzu TSAI - Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Province of ChinaLi-Tzu LI - Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan, Province of ChinaYuh-Lien CHEN - Institute of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, Taiwan, Province of ChinaChien-Jui CHENG - Graduate Institute of Medical Sciences and Department of Pathology, School of Medicine, Taipei Medical University, Taipei, Taiwan, Province of ChinaDao-Fu DAI - Section of Cardiology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan, Province of ChinaRuey-Bing YANG - Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Province of China
- Resource Type
- Journal article
- Publication Details
- Cardiovascular research, Vol.71(3), pp.486-495
- DOI
- 10.1016/j.cardiores.2006.04.010
- PMID
- 16753137
- NLM abbreviation
- Cardiovasc Res
- ISSN
- 0008-6363
- eISSN
- 1755-3245
- Publisher
- Oxford University Press; Oxford
- Language
- English
- Date published
- 2006
- Academic Unit
- Pathology; Iowa Neuroscience Institute; Radiation Oncology
- Record Identifier
- 9984047776302771
Metrics
20 Record Views