Journal article
Localization and regulation of renal Na+/myo-inositol cotransporter in diabetic rats
Kidney international, Vol.50(4), pp.1202-1211
10/1996
DOI: 10.1038/ki.1996.429
PMID: 8887279
Abstract
Localization and regulation of renal Na+/myo-inositol cotransporter in diabetic rats. We have examined theeffect of diabetes on sodium/myo-inositol cotransporter (SMIT) mRNA levels and myo-inositol content in the kidney to test the hypothesis that diabetes-induced changes in renal myo-inositol levels are due to the regulation of SMIT mRNA levels.In streptozotocin-induced diabetic rats, after 3, 7 and 28 days of diabetes, SMIT mRNA levels in the whole kidney were increased three- to fivefold, and remained increased by about twofold after six months of diabetes. Insulin treatment of diabetic rats normalized blood glucose levels and prevented the increase in SMIT mRNA levels. Treating diabetic rats with sorbinil, an aldose reductase inhibitor,corrected the abnormal accumulation of sorbitol but had no effect on the diabetes-induced increase in renal SMIT mRNA levels. The regional distribution of SMIT mRNA from normal rats showed a relative abundance in cortex, outer medulla, and inner medulla of 1.0:3.4:7.0. After seven days of diabetes, the levels of SMIT mRNA and myo-inositol content were significantly increased only in the outer medulla. In situ hybridization studies revealed that SMIT mRNA in the outer medulla was predominately localized to the medullary thick ascending limbs of Henle's loop and was not localized to any specific cell in the inner medulla. This distribution pattern was unchanged in diabetic rats. These studies show that diabetes causes an increase in renal SMIT mRNA, which is primarily localized to the outer medulla. Accumulation of myo-inositol by the thick ascending limb of Henle's loop may account for most of the increase caused by diabetes.
Details
- Title: Subtitle
- Localization and regulation of renal Na+/myo-inositol cotransporter in diabetic rats
- Creators
- Thomas J WieseKazumichi MatsushitaWilliam L LoweJohn B StokesMark A Yorek
- Resource Type
- Journal article
- Publication Details
- Kidney international, Vol.50(4), pp.1202-1211
- Publisher
- Elsevier Inc
- DOI
- 10.1038/ki.1996.429
- PMID
- 8887279
- ISSN
- 0085-2538
- eISSN
- 1523-1755
- Language
- English
- Date published
- 10/1996
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094772202771
Metrics
12 Record Views