Journal article
Localization of Ca 2+-binding proteins of rat cortex on two-dimensional gels–I. Identification of calmodulin and the b subunit of calcineurin
Neurochemistry international, Vol.12(2), pp.215-223
1988
DOI: 10.1016/0197-0186(88)90130-1
Abstract
At least three Ca
2+-binding proteins were detected in rat cortex by
45Ca
2+ autoradiography of two-dimensional electrophoretograms. The identities of two of these Ca
2+-binding proteins were determined to be calmodulin and the B subunit of calcineurin. The identification was based upon the following criteria: (1) co-localization on polyacrylamide gels with the appropriate purified proteins, (2) staining of nitrocellulose blots with specific antisera for calmodulin and calcineurin and (3) ability to bind Ca
2+. This information is useful in that it identifies two major brain proteins visible on silver-stained two-dimensional polyacrylamide gels. In addition, this data reveals the location of an unidentified Ca
2+-binding protein of molecular weight ∼ 18,000 Da and pI 5.4 on these gels.
Details
- Title: Subtitle
- Localization of Ca 2+-binding proteins of rat cortex on two-dimensional gels–I. Identification of calmodulin and the b subunit of calcineurin
- Creators
- Donna M. Santer - National Institute of Mental HealthWilliam E. Heydorn - United States Food and Drug AdministrationG.Joseph Creed - National Institute of Mental HealthDavid M. Jacobowitz - National Institute of Mental Health
- Resource Type
- Journal article
- Publication Details
- Neurochemistry international, Vol.12(2), pp.215-223
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/0197-0186(88)90130-1
- ISSN
- 0197-0186
- eISSN
- 1872-9754
- Language
- English
- Date published
- 1988
- Academic Unit
- Stead Family Department of Pediatrics; General Pediatrics and Adolescent Medicine
- Record Identifier
- 9984383923902771
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