Journal article
Localization of FtsL to the Escherichia coli septal ring
Molecular microbiology, Vol.31(2), pp.725-737
01/1999
DOI: 10.1046/j.1365-2958.1999.01213.x
PMID: 10027987
Abstract
In Escherichia coli, nine gene products are known to be essential for assembly of the division septum. One of these, FtsL, is a bitopic membrane protein whose precise function is not understood. Here we use fluorescence microscopy to study the subcellular localization of FtsL, both in a wild‐type strain and in a merodiploid strain that expresses a GFP–FtsL fusion protein. We show that FtsL localizes to the cell septum where it forms a ring analogous to the cytoplasmic FtsZ ring. FtsL localization is dependent upon the function of FtsZ, FtsA and FtsQ, but not FtsI. In a reverse approach, we use fusions of green fluorescent protein (GFP) to FtsZ, FtsA and ZipA to show that these proteins localize to the division site in an FtsL‐independent fashion. We propose that FtsL is a relatively late recruit to the ring structure that mediates septation.
Details
- Title: Subtitle
- Localization of FtsL to the Escherichia coli septal ring
- Creators
- Jean‐Marc GhigoDavid S WeissJoseph C ChenJustin C YarrowJon Beckwith
- Resource Type
- Journal article
- Publication Details
- Molecular microbiology, Vol.31(2), pp.725-737
- Publisher
- Blackwell Science Ltd; Oxford BSL
- DOI
- 10.1046/j.1365-2958.1999.01213.x
- PMID
- 10027987
- ISSN
- 0950-382X
- eISSN
- 1365-2958
- Number of pages
- 13
- Language
- English
- Date published
- 01/1999
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001109802771
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