Localization of MYOC transcripts in human eye and optic nerve by in situ hybridization

Ruth E Swiderski; Jean L Ross; John H Fingert; Abbot F Clark; Wallace L M Alward; Edwin M Stone; Val C Sheffield

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Localization of MYOC transcripts in human eye and optic nerve by in situ hybridization

Journal article

Peer reviewed

Localization of MYOC transcripts in human eye and optic nerve by in situ hybridization

Ruth E Swiderski, Jean L Ross, John H Fingert, Abbot F Clark, Wallace L M Alward, Edwin M Stone and Val C Sheffield

Investigative ophthalmology & visual science, Vol.41(11), pp.3420-3428

10/2000

PMID: 11006234

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Abstract

To evaluate MYOC (myocilin) gene expression at the RNA level in normal intact human eyes and optic nerve using in situ hybridization. Normal human eyes and optic nerves from donors 62 to 83 years of age with no history of glaucoma were fixed, embedded in paraffin, and sectioned. Sections were hybridized with (35)S-labeled sense and antisense riboprobes derived from a full-length MYOC cDNA. High levels of MYOC expression were observed throughout the trabecular meshwork as well as in the most anterior nonfiltering meshwork (Schwalbe's line), in the scleral spur, and in the endothelial lining of Schlemm's canal. MYOC transcripts were also detected in the anterior corneal stroma, in the ciliary muscle, beneath the anterior border of the iris, in the iris stroma, and in the sclera. Expression in the retrolaminar region of the optic nerve was present in the pial septa that divide the nerve fiber bundles, in the perivascular connective tissue surrounding the central retinal vessels, and in the dura mater, arachnoid, and pia mater of the meningeal sheath surrounding the optic nerve. MYOC gene expression in the trabecular meshwork, Schlemm's canal, scleral spur, and ciliary muscle indicates a structural or functional role for myocilin in the regulation of aqueous humor outflow that may influence intraocular pressure. MYOC expression in the optic nerve suggests that changes in the structural, metabolic, or neurotropic support of the optic nerve may influence its susceptibility to glaucomatous damage.

Gene Expression

Cytoskeletal Proteins

Glycoproteins - genetics

Sclera - metabolism

Humans

Middle Aged

Muscle, Smooth - metabolism

Ciliary Body - metabolism

Glycoproteins - biosynthesis

RNA, Messenger - biosynthesis

Blotting, Northern

In Situ Hybridization

Endothelium - metabolism

Trabecular Meshwork - metabolism

Aged, 80 and over

Aged

Eye Proteins - genetics

Eye Proteins - biosynthesis

Optic Nerve - metabolism

Details

Title: Subtitle: Localization of MYOC transcripts in human eye and optic nerve by in situ hybridization
Creators: Ruth E Swiderski - Department of Pediatrics, University of Iowa, Iowa City 52242, USA
Jean L Ross
John H Fingert
Abbot F Clark
Wallace L M Alward
Edwin M Stone
Val C Sheffield
Resource Type: Journal article
Publication Details: Investigative ophthalmology & visual science, Vol.41(11), pp.3420-3428
PMID: 11006234
NLM abbreviation: Invest Ophthalmol Vis Sci
ISSN: 0146-0404
eISSN: 1552-5783
Publisher: United States
Grant note: R01 EY-10564 / NEI NIH HHS
Language: English
Date published: 10/2000
Academic Unit: Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Medical Genetics and Genomics; Ophthalmology and Visual Sciences
Record Identifier: 9983980020702771

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