Journal article
Localization of a biologically important epitope on toxic-shock-syndrome toxin-1
The Journal of infectious diseases, Vol.158(3), pp.549-555
09/1988
DOI: 10.1093/infdis/158.3.549
PMID: 2457635
Abstract
A monoclonal antibody, designated B-14, inhibits the nonspecific T lymphocyte mitogenicity of toxic-shock-syndrome toxin-1 (TSST-1), and the antibody binds to an internal cyanogen bromide (CNBr) fragment (Mr, 14,000) of the toxin. The epitope recognized by B-14 was further localized to include a decapeptide at the NH2-terminus of the CNBr fragment. The decapeptide inhibited the ELISA and western blot reactivity of B-14 with TSST-1, although it was approximately 10,000-fold less effective than the native toxin. The peptide also inhibited the capacity of B-14 to block TSST-1-induced mitogenicity. A conjugate, consisting of decapeptide4-ovalbumin, was used to hyperimmunize three rabbits. Serum from these rabbits reacted specifically with intact TSST-1 in ELISA and western blots and partially neutralized toxin mitogenicity; however, the serum did not prevent fever and enhancement of susceptibility to endotoxin shock typically seen in rabbits after administration of TSST-1.
Details
- Title: Subtitle
- Localization of a biologically important epitope on toxic-shock-syndrome toxin-1
- Creators
- Brian G Murphy - Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455-0312Barry N KreiswirthRichard P NovickPatrick M Schlievert
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.158(3), pp.549-555
- Publisher
- United States
- DOI
- 10.1093/infdis/158.3.549
- PMID
- 2457635
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Grant note
- AI-22159 / NIAID NIH HHS
- Language
- English
- Date published
- 09/1988
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001229602771
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