Localization of an immunologically functional region of the streptococcal superantigen pepsin-extracted fragment of type 5 M protein

Bo Wang; Patrick M Schlievert; Ahmed Osama Gaber; Malak Kotb

doi:10.4049/jimmunol.151.3.1419

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Localization of an immunologically functional region of the streptococcal superantigen pepsin-extracted fragment of type 5 M protein

Journal article

Peer reviewed

Localization of an immunologically functional region of the streptococcal superantigen pepsin-extracted fragment of type 5 M protein

Bo Wang, Patrick M Schlievert, Ahmed Osama Gaber and Malak Kotb

The Journal of immunology (1950), Vol.151(3), pp.1419-1429

08/01/1993

DOI: 10.4049/jimmunol.151.3.1419

PMID: 8335938

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Abstract

Ten overlapping synthetic peptides, spanning the entire amino acid sequence of pepsin-extracted type 5 M protein, (pep M5), were used to delineate regions within this molecule that are important for the mitogenic activity of this streptococcal superantigen. Two of the overlapping peptides, SM5-8 and SM5-10, blocked the response of T cells to pep M5 in a dose-dependent manner. Neither peptide was toxic to T cells, but SM5-8 exhibited nonspecific inhibition of the T-cell response to either superantigen or polyclonal mitogens. In contrast, the inhibition by SM5-10 was pep M5-specific. Analysis of TCR V beta gene usage of the pep M5 response in the presence and absence of SM5-10 revealed that this peptide preferentially blocks the expansion of pep M5-specific V beta elements. The degree of inhibition of V beta expansion varied from one individual to the other, reflecting their TCR repertoire. SM5-10 did not significantly inhibit the response of T cells to other streptococcal superantigens. The data suggest that the region represented by SM5-10 of the pep M5 is important for superantigenic activity. In addition, the ability of this synthetic peptide to inhibit the response to pep M5 provides evidence that the mitogenic response is indeed mediated by this superantigen and is not a result of contamination with other streptococcal superantigens.

Amino Acid Sequence

Bacterial Outer Membrane Proteins

Antigens, Bacterial - chemistry

Peptides - chemistry

Protein Structure, Secondary

Streptococcus pyogenes - immunology

Humans

Pepsin A

Peptides - immunology

Bacterial Proteins - chemistry

Molecular Sequence Data

Receptors, Antigen, T-Cell, alpha-beta - immunology

Antigen-Presenting Cells - immunology

Sequence Alignment

T-Lymphocytes - immunology

Bacterial Proteins - immunology

In Vitro Techniques

Carrier Proteins

Details

Title: Subtitle: Localization of an immunologically functional region of the streptococcal superantigen pepsin-extracted fragment of type 5 M protein
Creators: Bo Wang - University of Tennessee Health Science Center
Patrick M Schlievert
Ahmed Osama Gaber
Malak Kotb
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.151(3), pp.1419-1429
DOI: 10.4049/jimmunol.151.3.1419
PMID: 8335938
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: HL36611 / NHLBI NIH HHS GM-38530 / NIGMS NIH HHS
Language: English
Date published: 08/01/1993
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001118802771

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