Localization of biologically important regions on toxic shock syndrome toxin 1

Debra L Murray; Cathleen A Earhart; David T Mitchell; Douglas H Ohlendorf; Richard P Novick; Patrick M Schlievert

doi:10.1128/IAI.64.1.371-374.1996

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Localization of biologically important regions on toxic shock syndrome toxin 1

Journal article

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Peer reviewed

Localization of biologically important regions on toxic shock syndrome toxin 1

Debra L Murray, Cathleen A Earhart, David T Mitchell, Douglas H Ohlendorf, Richard P Novick and Patrick M Schlievert

Infection and immunity, Vol.64(1), pp.371-374

01/1996

DOI: 10.1128/IAI.64.1.371-374.1996

PMCID: PMC173772

PMID: 8557369

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Abstract

Toxic shock syndrome toxin 1 (TSST-1) contains a long central alpha helix that forms the base of two grooves on opposite sides of the molecule. Previous studies indicated that residues 132, 135, and 140 along the back of the central alpha helix are important in the biological activities. We made mutations of additional central alpha-helix residues exposed along this groove on the back of TSST-1. The proteins were purified, shown not to have gross alteration in structure, and tested for both superantigenicity and ability to elicit lethal TSS, using the superantigenicity, likely to because of alteration in T-cell receptor binding. Mutants H135A, Q136A, and E132K/ Q136K lost the ability to induce lethal TSS. The mutant Q136A was most increasing because it was superantigenic, yet nonlethal.

Details

Title: Subtitle: Localization of biologically important regions on toxic shock syndrome toxin 1
Creators: Debra L Murray - Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455, USA
Cathleen A Earhart - Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455, USA
David T Mitchell - Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455, USA
Douglas H Ohlendorf - Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455, USA
Richard P Novick - Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455, USA
Patrick M Schlievert - Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455, USA
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.64(1), pp.371-374
DOI: 10.1128/IAI.64.1.371-374.1996
PMID: 8557369
PMCID: PMC173772
ISSN: 0019-9567
eISSN: 1098-5522
Language: English
Date published: 01/1996
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001119702771

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